Chiral NIR-Ⅱ fluorescent Ag_(2)S quantum dots with stereospecific biological interactions and tumor accumulation behaviors  

手性NIR-Ⅱ荧光Ag_(2)S量子点的生物相互作用及其肿瘤靶向特性

作　　者：Shaohua Qu Qian Jia Zheng Li Zhongliang Wang Li Shang 屈少花;贾茜;李正;王忠良;尚利(State Key Laboratory of Solidification Processing,School of Materials Science and Engineering,Northwestern Polytechnical University(NPU),Xi’an 710072,China;Lab of Molecular Imaging and Translational Medicine(MITM),Engineering Research Center of Molecular&Neuroimaging,Ministry of Education,School of Life Science and Technology,Xidian University,Xi’an 710126,China;Academy of Advanced Interdisciplinary Research,Xidian University,Xi’an 710071,China)

机构地区：[1]State Key Laboratory of Solidification Processing,School of Materials Science and Engineering,Northwestern Polytechnical University(NPU),Xi’an 710072,China [2]Lab of Molecular Imaging and Translational Medicine(MITM),Engineering Research Center of Molecular&Neuroimaging,Ministry of Education,School of Life Science and Technology,Xidian University,Xi’an 710126,China [3]Academy of Advanced Interdisciplinary Research,Xidian University,Xi’an 710071,China

出　　处：《Science Bulletin》2022年第12期1274-1283,M0004,共11页科学通报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2017YFC1309100 and 2017YFA0205200);the National Natural Science Foundation of China(21804104 and 91959124);the Research Fund of the State Key Laboratory of Solidification Processing(NPU,2020-QZ-01);the Fundamental Research Fund for the Central University(3102019jcc005);the Youth Innovation Team of Shaanxi Universities,Natural Science Foundation of Shaanxi Province(2020PT-020)。

摘　　要：Near-infrared Ⅱ(NIR-Ⅱ)fluorescent nanoprobes hold great potential for biomedical applications.Elucidating the relationship between surface properties of NIR-Ⅱ nanoprobes and their biological behaviors is particularly important for future probe design and their performance optimization.Despite the rapid development of NIR-Ⅱ nanoprobes,the distinct role of surface chirality on their biological fates has rarely been exploited.Herein,chiral NIR-Ⅱ fluorescent Ag_(2)S quantum dots(QDs)are synthesized to investigate the relationship between their chirality and biological functions at both in vitro and in vivo levels.D-/L-Ag_(2)S QDs exhibit significant differences on their interactions with serum proteins,which further affect the cellular uptake.As a result,D-Ag_(2)S QDs can be internalized with higher efficiency(over 2-fold)than that of L-Ag_(2)S QDs.Moreover,in vivo studies reveal that the chirality determines the primary localization of these chiral QDs,where a more efficient renal elimination of D-Ag_(2)S QDs was observed than that of L-Ag_(2)S QDs.Importantly,D-Ag_(2)S QDs show preferential accumulation in tumor region than that of L-Ag_(2)S QDs in orthotopic kidney tumor model,which points out a new avenue of enhancing targeting capabilities of nanoprobes by engineering their surface chirality.手性配体不仅可以赋予纳米探针独特的物化性质,而且会影响其生物学行为.因此,明确手性在纳米-生物界面的独特作用对其生物医学应用具有重要的科学意义.本文首次合成了手性的近红外二区荧光Ag_(2)S量子点,并结合多种分析技术系统探究了其生物学效应.研究结果表明,Ag_(2)S量子点与蛋白质和细胞的相互作用以及活体水平的代谢行为均表现出显著的手性依赖性,而且D-Ag_(2)S量子点还呈现出对肾肿瘤的靶向富集的特性.本文的研究结果有助于进一步理解纳米-生物界面的手性效应,并为发展新型肿瘤靶向探针提供了新颖的设计思路.

关 键 词：CHIRALITY NIR-Ⅱprobes Ag_(2)S quantum dots Protein corona Tumor targeting 

分 类 号：R73[医药卫生—肿瘤] TB383.1[医药卫生—临床医学]