响应面法优化地黄多糖的提取工艺及抗氧化活性分析  

Optimization of extraction and antioxidant activity of crude polysaccharides from Rehmannia glutinosa Libosh.by response surface methodology

作　　者：周加林[1] 张涵 岳义民 梁蓝元 李传球 舒尊鹏 王毅 ZHOU Jialin;ZHANG Han;YUE Yimin;LIANG Lanyuan;LI Chuanqiu;SHU Zunpeng;WANG Yi(Beijing Sixth Hospital Traditional Chinese Medicine Pharmacy,Beijing 100007,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Pharmacy,Jiamusi University,Jiamusi 154007,China)

机构地区：[1]北京市第六医院中药房,北京100007 [2]广东药科大学中药学院,广东广州510006 [3]佳木斯药学院,黑龙江佳木斯154007

出　　处：《广东药科大学学报》2022年第4期64-71,共8页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金面上项目(82174043);国家自然科学基金专项项目(82141211);广东省自然科学基金面上项目(2021A1515011697);佛山高新区高技术产业化创业团队项目(2120197000214);佛山市顺德区引进创新创业团队项目(2130218004133)。

摘　　要：目的通过响应面法优化地黄多糖的提取工艺并考察其抗氧化活性。方法使用水提醇沉法提取地黄多糖,在单因素试验的基础上,以提取时间、料液比和提取温度为自变量,以地黄多糖提取率为响应值,采用响应面法优化地黄多糖的提取工艺。对比Sevage法与三氯乙酸法脱蛋白的效果,并通过测定地黄多糖对DPPH自由基、羟基自由基的清除作用及Fe3+还原力评估其体外抗氧化活性。结果地黄多糖的最佳提取工艺条件为:提取时间100 min,料液比1∶60(g∶mL),温度70℃;在最佳条件下地黄多糖提取率可达7.09%,相对误差为0.29%。脱蛋白结果表明,三氯乙酸法较Sevage法更适于地黄多糖脱蛋白。多糖的抗氧化试验结果显示,在多糖质量浓度为1.0 mg/mL时,DPPH自由基、羟基自由基的最大清除率分别为72.44%、72.97%,还原能力评估吸光度值为0.5904。结论优化了地黄多糖的提取工艺,地黄多糖有较好的体外抗氧化活性,为地黄多糖的进一步开发利用提供了理论支持。Objective Response surface methodology(RSM)was used to optimize the extraction process of Rehmannia glutinosa Libosh.polysaccharide(RGP)and its antioxidant activity was evaluated.Methods RGP was extracted by water extraction and alcohol precipitation.A RSM experiment was used to optimize the extraction procedure of RGP based on a single-factor experiment,with extraction time,solid-liquid ratio and temperature as independent variables and the extraction yield as response value.The deproteinization effects of Sevage method and trichloroacetic acid(TCA)method were compared,and the in vitro antioxidant activity of RGP was assessed by the scavenging activity on 1,1-diphenyl-2-trimethylphenylhydrazine(DPPH)radical,hydroxyl radical and Fe3+reducing power.Results The optimal process conditions for RGP were as follows:extraction time of 100 min,solid-liquid ratio of 1∶60(g∶mL)and extraction temperature of 70℃.The extraction rate of RGP was 7.09%with relative error of 0.29%under the best extraction conditions.TCA method was more suitable for RGP deproteinization compared with Sevage method.The results of antioxidant experiments of RGP showed that when the concentration of RGP was 1.0 mg/mL,the maximum scavenging rate of DPPH radical,hydroxyl radical and the absorbance values corresponding to their reducing ability were 72.44%,72.97%,and0.5904,respectively.Conclusion The extraction process of RGP was optimized,and RGP had good antioxidant activity,which could provide a theoretical support for its further development and utilization.

关 键 词：地黄多糖 响应面分析法 提取工艺 抗氧化活性 

分 类 号：R282.2[医药卫生—中药学]