马铃薯PYL基因家族的全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of potato PYL gene family

作　　者：贾小霞[1,2] 齐恩芳 马胜[1,2] 黄伟 郑永伟[1,2] 白永杰 文国宏[1,2] JIA Xiao-Xia;QI En-Fang;MA Sheng;HUANG Wei;ZHENG Yong-Wei;BAI Yong-Jie;WEN Guo-Hong(Potato Research Institute of Gansu Academy of Agricultural Sciences/Gansu Engineering Laboratory of Potato Germplasm resources Innovation,Lanzhou 730070,Gansu,China;National Germplasm Resources Agricultural Experimental Station,Weiyuan 748201,Gansu,China)

机构地区：[1]甘肃省农业科学院马铃薯研究所/甘肃省马铃薯种质资源创新工程实验室,甘肃兰州730070 [2]国家种质资源渭源观测实验站,甘肃渭源748201

出　　处：《作物学报》2022年第10期2533-2545,共13页Acta Agronomica Sinica

基　　金：甘肃省自然科学基金重点项目(21JR7RA728);甘肃省农业科学院科技创新专项(2020GAAS16);甘肃省农业科学院生物技术育种专项(2020GAAS10);国家自然科学基金项目(31560412,31860401);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09-P06)资助。

摘　　要：作为关键信号分子,脱落酸(ABA)通过其核心信号通路PYLs-PP2Cs-SnRK2s广泛调控植物的生长发育和逆境响应过程,PYLs蛋白作为ABA信号传导的核心组件,在ABA信号传导中发挥着不可替代的作用。为探究PYLs(PYR/PYL/RCARs)基因在马铃薯中的进化以及表达模式,本研究从马铃薯全基因组‘DM-v 6.1’共鉴定到17个StPYLs基因,并对其分布、蛋白理化性质、系统进化、基因结构特征以及基因表达模式进行了分析。结果表明,17个StPYLs基因不均匀分布在8条染色体上,其氨基酸大小在163~231 aa之间,等电点在4.5~8.6之间,相对分子量在18.71~25.29 kD之间。根据基因结构和蛋白的系统发育特征,StPYL家族成员共分为3个亚组,motif 1存在于本家族所有基因中,说明它在StPYLs的进化过程中较为保守。基因表达模式分析表明,StPYL家族成员具有明显的组织表达特异性,且除StPYL1在外源激素(BAP、ABA和IAA)和非生物胁迫(高温、盐和干旱)下均上调表达外,其余基因存在功能分化,不同胁迫下的表达模式各异。本研究结果为进一步阐明StPYLs基因在马铃薯中的功能奠定了理论基础。As a key signalling molecule,abscisic acid(ABA)extensively regulates plant growth and development,and stress response processes through its core signalling pathway PYLs-PP2Cs-SnRK2s.As a core component of ABA signalling transduction,PYLs protein plays an irreplaceable role.In order to explore the evolution and expression pattern of PYL(PYR/PYL/RCARs)genes in potato,17 PYL genes were identified from the whole potato genome‘DM-v 6.1’,and their distribution,protein physical and chemical properties,system evolution,structural characteristics,and the relative expression patterns were analysed.The results showed that the 17 StPYL genes were unevenly distributed on 8 chromosomes,with amino acids of 163–231 aa,isoelectric points of 4.5–8.6,and relative molecular weights of 18.71–25.29 kD.According to the gene structure and protein phylogenetic characteristics,the members of the StPYL family were divided into three subgroups.Motif 1 existed in all genes in this family,indicating that it was relatively conserved in the evolution of StPYLs.Gene expression patterns showed that the members of the StPYL family had obvious tissue expression specificity,and except for the up-regulation of StPYL1 under exogenous hormones(BAP,ABA,and IAA)and abiotic stress(high temperature,salt and drought),the rest of the StPYL genes were functionally differentiated and their relative expression patterns were different under different stresses.The results of this study lay a theoretical foundation for further elucidating the function of StPYLs gene in potato.

关 键 词：马铃薯 PYL基因家族 脱落酸 基因表达分析 

分 类 号：S532[农业科学—作物学]