桉木预水解液中半纤维素多糖分离纯化及抗氧化活性研究  

Study on Separation, Purification and Antioxidant Activities of Hemicellulose Polysaccharides from Eucalyptus Pre-hydrolysate

作　　者：李学秀 刘海棠[1] 安永贞 刘婧[1] 陈琳 金鑫 LI Xuexiu;LIU Haitang;AN Yongzhen;LIU Jing;CHEN Lin;JIN Xin(China Light Industry Key Lab of Papermaking and Biorefinery,Tianjin Key Lab of Pulp and Paper,Tianjin University of Science&Technology,Tianjin,300457)

机构地区：[1]中国轻工业造纸与生物质精炼重点实验室,天津科技大学天津市制浆造纸重点实验室,天津300457

出　　处：《中国造纸》2022年第4期30-37,共8页China Pulp & Paper

基　　金：国家自然科学基金青年科学基金项目(31700516);天津市制浆造纸重点实验室开放基金(202005)。

摘　　要：采用醇沉法从桉木预水解液中提取半纤维素粗多糖(PHLP),并利用DEAE-650M离子交换柱对其进行分离纯化,对纯化得到的中性多糖PHLP-1和酸性多糖PHLP-2两种组分进行分析。单糖组分结果表明,PHLP-1和PHLP-2均由阿拉伯糖、木糖、半乳糖、甘露糖及葡萄糖组成,摩尔比分别为0.09∶1∶0.13∶0.07∶0.35和0.1∶1∶0.03∶0.09∶0.08。红外光谱测定表明,两种组分均具有多糖的特征吸收峰。扫描电子显微镜表明,PHLP-1和PHLP-2呈无序杂乱的结构形态。抗氧化实验结果表明,两种纯化的多糖组分均具有一定的抗氧化性且存在量效关系,抗氧化能力强弱顺序为:PHLP-2>PHLP-1>PHLP。The hemicellulose polysaccharides(PHLP)were extracted from the eucalyptus wood pre-hydrolysate(PHL)by alcohol precipitation,and were separated and purified by DEAE-650M ion exchange column. Meanwhile,the two purified components neutral polysaccharide PHLP-1 and acidic polysaccharide PHLP-2 were analyzed. The results of monosaccharide components showed that both PHLP-1 and PHLP-2 were composed of arabinose,xylose,galactose,mannose and glucose,with a molar ratio of 0. 09∶1∶0. 13∶0. 07∶0. 35 and0. 1∶1∶0. 03∶0. 09∶0. 08. The infrared spectroscopy showed that both components had characteristic absorption peaks of polysaccharides. The scanning electron microscopy analysis showed that the structures of PHLP-1 and PHLP-2 were disordered and messy. The results of antioxidant experiments showed that the two purified polysaccharides had certain antioxidant properties and there was a dose-effect relationship. The order of antioxidant ability was:PHLP-2>PHLP-1>PHLP.

关 键 词：预水解液 多糖 分离纯化 抗氧化活性 

分 类 号：TS722[轻工技术与工程—制浆造纸工程] TQ353.7[化学工程]