海甘蓝(Crambe abyssinica)叶绿体基因组特征及其系统发育研究  

Characteristics of Crambe abyssinica Chloroplast Genome and Its Phylogenetic Relationship in Brassicaceae

作　　者：钱方 高作敏 胡利娟 王洪程 QIAN Fang;GAO Zuo-min;HU Li-juan;WANG Hong-cheng(School of Life Sciences,Guizhou Normal University,Guiyang 550001)

机构地区：[1]贵州师范大学生命科学学院,贵阳550001

出　　处：《生物技术通报》2022年第6期174-186,共13页Biotechnology Bulletin

基　　金：贵州省科学计划项目(QKHJC(2020)1Y102)。

摘　　要：海甘蓝(Crambe abyssinica)为十字花科(Brassicaceae)海甘蓝属(Crambe)植物,其种子富含芥酸,被视为一种重要的可再生工业油用资源。本研究基于Illumina HiSeq 测序数据从头组装了(de novo)海甘蓝的叶绿体(chloroplast,cp)基因组,并分析其在十字花科植物中的系统发育位置。结果表明,海甘蓝叶绿体基因组大小为153 754 bp,呈典型的四分体结构;其包含111个基因,包括78个编码基因、29个tRNA基因以及4个rRNA基因。通过密码子偏好性分析,海甘蓝叶绿体基因组中末端为A/U的密码子使用存在偏倚。SSR及长重复片段分析检测到41个重复序列和59个SSR位点。与近缘种比较,发现其叶绿体基因组序列高度保守,尤其蛋白质编码序列相似度极高。同义与非同义核苷酸替代率分析表明,除ndhB和ycf2基因外,其余编码基因普遍存在纯化选择压力。此外,系统发育分析发现海甘蓝与芸薹属重要的经济作物亲缘关系密切,与白芥属植物形成姊妹类群。这为海甘蓝与芸薹属植物的种间杂交提供了遗传支撑,同时也为阐明海甘蓝的进化史提供了重要的分子信息。Crambe abyssinica,belonging to Crambe genus in Brassicaceae,has been used as a potentially renewable industrial oil resource because of its high natural erucic acid content in seed.Here,the chloroplast(cp)genome of C.abyssinica was de novo assembled by Illumina Platform,and its phylogenetic relationship was detected based on the cp genome in Brassicaceae.The cp genome of C.abyssinica had a quadripartite structure of 153754 bp in length,which were annotated a total of 111 unigenes,including 78 protein-coding genes,29 tRNA genes and 4 rRNA genes.Codon usage showed a bias of A/U-ending codons in the cp genome of C.abyssinica.A total of 41 repeat structures and 59 SSRs(simple sequence repeats)were detected in this cp genome.Comparative analysis showed that the genome sequence was conserved and the protein-coding sequence had high similarity.Synonymous and non-synonymous analysis detected strong positive selection exerted on the ndhF and ycf2 gene in the cp genome of C.abyssinica.Moreover,phylogenetic analysis showed that C.abyssinica was closely related to Brassica and formed a sister group with Sinapis species.This suggests that it is possible to transfer favorable genes from C.abyssinica to other Brassicaceae plants by distant hybridization,and also provide important molecular information for elucidating the evolutionary history of C.abyssinica.

关 键 词：海甘蓝 叶绿体基因组 比较分析 系统发育 

分 类 号：Q943[生物学—植物学]