磷酸甜菜碱对产黑色素短梗霉发酵产聚苹果酸的影响  

Effect of betaine phosphate on polymalic acid fermentationby Aureobasidium melanogenum

作　　者：杨东霖 李佳谦 鲁亮 王舸楠 王纪 赵廷彬 殷海松[3] 乔长晟[1,2,4,5] YANG Donglin;LI Jiaqian;LU Liang;WANG Genan;WANG Ji;ZHAO Tingbin;YIN Haisong;QIAO Changsheng(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Huizhi Baichuan Bioengineering Co.,Ltd.,Tianjin 300457,China;College of Bioengineering,Tianjin Modern Vocational Technology College,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China;Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津慧智百川生物工程有限公司,天津300457 [3]天津现代职业技术学院生物工程学院,天津300457 [4]工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室,天津300457 [5]天津市微生物代谢与发酵过程控制技术工程中心,天津300457

出　　处：《食品与发酵工业》2022年第13期33-41,共9页Food and Fermentation Industries

基　　金：天津市科委基金项目(21YDTPJC00140,18YFZCNC01140)。

摘　　要：聚苹果酸是一种主要由微生物合成的有机生物高分子材料,可应用于生物制药与可降解材料等领域。该研究以产黑色素短梗霉(Aureobasidium melanogenum)为研究对象,探究磷酸甜菜碱对菌体发酵聚苹果酸的影响。结果发现:磷酸甜菜碱最适添加量为0.9 g/L,5 L罐实验在通风量为8 L/min、恒定转速450 r/min的最适条件下聚苹果酸产量最后可达到53.10 g/L远高于空白组(17.29 g/L)。通过转录组分析发现,菌体的己糖激酶与磷酸烯醇式丙酮酸羧激酶等关键酶表达量提高,说明菌体对糖的利用能力与聚苹果酸合成的能力增强,该研究为聚苹果酸工业化生产提供了一种可行方案,为磷酸甜菜碱在其他发酵领域中的应用提供了参考。Polymalic acid(PMLA)is an organic biopolymer material mainly synthesized by microorganisms,which can be used in biopharmaceuticals,degradable materials and other industries.In this study,the effect of betaine phosphate on the fermentation of polymalic acid by Aureobasidium melanogenum was investigated.The results showed that,under the optimum conditions of 8 L/min ventilation,450 r/min constant speed,and the optimum concentration of betaine phosphate 0.9 g/L,the final polymalic acid yield was 53.10 g/L,while that in blank group was 17.29 g/L.Transcriptome analysis showed that the expression levels of key enzymes such as hexose kinase and phosphoenolpyruvate carboxykinase were increased.This study provides a feasible solution for the industrial production of polymalic acid and provides a reference for the application of betaine phosphate in other fermentation fields.

关 键 词：产黑色素短梗霉 聚苹果酸 磷酸甜菜碱 工艺优化 转录组学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ317