壳聚糖-核桃多肽脂质体的制备及表征  

Preparation and characterization of chitosan-coated nanoliposome loaded with walnut(Juglans regia L.)polypeptide

作　　者：郝静 涂心怡 曹诗诺 汪涛 王丰俊 HAO Jing;TU Xinyi;CAO Shinuo;WANG Tao;WANG Fengjun(College of Biological Sciences and Biotechnology,Beijing Forestry University,Key Laboratory of Forestry Food Processing and Safety,Beijing 100083,China)

机构地区：[1]北京林业大学生物科学与技术学院,林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品与发酵工业》2022年第13期135-140,共6页Food and Fermentation Industries

基　　金：北京市科技重大专项(Z181100009318012);核桃产业国家创新联盟(NAWI)资助项目。

摘　　要：为了提高核桃多肽的消化稳定性和贮藏稳定性,构建壳聚糖-脂质体载体进行包载,通过单因素试验探究工艺参数对壳聚糖-核桃多肽脂质体包封效果的影响。结果显示,当m(多肽)∶m(胆固醇)∶m(吐温-80)∶m(卵磷脂)=4∶1∶2∶9,壳聚糖质量分数为0.4%,超声波处理6 min时,壳聚糖-核桃多肽脂质体的包封效果最佳,此时壳聚糖-核桃多肽脂质体的包封率为(76.13±0.84)%,粒径为(80.30±0.35)nm,多分散系数为(0.25±0.01),电位为(+6.33±0.23)mV。采用透射电镜观察样品的微观形态,壳聚糖-核桃多肽脂质体呈明显的囊泡状结构,具有脂质体的形态特性。In order to improve the digestion and storage stability of walnut polypeptide,chitosan-coated nanoliposome carrier was constructed for encapsulation.The influence of technological parameters on the encapsulation effect of chitosan-coated nanoliposome loaded with walnut polypeptide was investigated by single factor experiment.The results showed that under the following conditions,m(polypeptide)∶m(cholesterol)∶m(Tween-80)∶m(lecithin)=4∶1∶2∶9 and the concentration of chitosan was 0.4%follwed the ultrasonic treatment for 6 min,the chitosan-coated nanoliposome loaded with walnut polypeptide had the best encapsulation efficiency.The encapsulation efficiency of chitosan-coated nanoliposome loaded with walnut polypeptide was(76.13±0.84)%,the particle size was(80.30±0.35)nm,polydispersity index was(0.25±0.01),and the potential was(+6.33±0.23)mV.The morphology of the sample was observed by transmission electron microscope.The results showed that the chitosan-coated nanoliposomes loaded with walnut polypeptide showed vesicular structure and the morphological characteristics of liposomes.

关 键 词：核桃多肽 脂质体 壳聚糖 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]