肝癌调控细胞凋亡抑制因子1的表达及其对细胞增殖和凋亡的影响  

Study on the expression of TP53 regulation of apoptosis inhibitor 1 in hepatoma cells and its effect on proliferation and apoptosis

作　　者：杨刚[1,2] 王冠[3] 熊永福[2] 李芸 雷蓉[3] 柳弥[3] YANG Gang;WANG Guan;XIONG Yongfu(Department of Hepatobiliary Surgery,Pingchang County People’s Hospital,Sichuan,Pingchang 636400,China)

机构地区：[1]四川省平昌县人民医院肝胆外科,636400 [2]川北医学院附属医院肝胆外科,川北医学院肝胆胰肠研究所 [3]川北医学院附属医院健康管理中心

出　　处：《临床外科杂志》2022年第5期471-474,共4页Journal of Clinical Surgery

摘　　要：目的探究TP53调控细胞凋亡抑制因子1(TRIAP1)对肝癌细胞Huh7和HepG2增殖和侵袭能力的影响。方法本研究首先使用TCGA数据库分析肿瘤组织和正常组织中TRIAP1的表达差异,分析TRIAP1表达与肝癌病人预后的相关性。通过在肝癌细胞Huh7和HepG2中转染siRNA及siControl构建敲低TRIAP1的细胞系,Western blot验证转染TRIAP1 siRNA的敲低效率及其他相关蛋白水平。Capase3/7活性细胞凋亡试剂盒、Annex inV/PI法检测在肝癌细胞中敲低TRIAP1对肝癌细胞凋亡的影响,CCK8检测TRIAP1对肝癌细胞增殖的影响。结果TCGA数据库分析显示,肝癌细胞中TRIAP1表达上调,且与肝癌病人预后呈负相关。在肝癌细胞Huh7和HepG2中敲低TRIAP1实验组TRIAP1的表达比对照组表达明显下降,敲低TRIAP1后实验组中p21在mRNA和蛋白表达水平均出现上调,敲低TRIAP1实验组的增殖能力显著下降,而凋亡细胞数量明显增加。结论TRIAP1在肝癌细胞中表达上调,负调控细胞周期相关基因p21的表达调节肿瘤细胞周期,抑制TRIAP1可显著上调p21,抑制肝癌细胞增殖能力,促进肝癌细胞凋亡进程。Objective To investigate the effects of TP53 regulation of apoptosis inhibitor 1(TRIAP1)on the proliferation and invasion ability of hepatoma cells Huh7 and HepG2.Methods In this study,the difference of TRIAP1 expression in tumor tissues and normal tissues was analyzed using TCGA database,and the correlation between TRIAP1 and prognosis of HCC patients was analyzed.TRIAP1 knockdown cell lines were constructed by transfection with siRNA and control group.Western blot was used to verify knockdown efficiency of TRIAP1 siRNA transfection and the levels of other related proteins.The effect of TRIAP1 on the apoptosis of HCC cells was detected by Capase 3/7 active cell apoptosis kit and Annex INV/PI assay,and the effect of TRIAP1 on the proliferation of HCC cells was detected by CCK8.Results TCGA database analysis showed that TRIAP1 expression was up-regulated in HCC cells,and was negatively correlated with prognosis of HCC patients.siTRIAP1 was transfected into Huh7 and HepG2 to downregulate TRIAP1 expression..The mRNA and protein expression levels of p21 in the TRIAP1 knockdown group were significantly up-regulated,and the proliferation ability of the TRIAP1 knockdown group was decreased,while the number of apoptotic cells was increased.Conclusion The expression of TRIAP1 is up-regulated in HCC cells,and the expression of cell cycle related p21 expression is decreased and then regulate the tumor cell cycle.Inhibition of TRIAP1 can significantly up-regulate p21,inhibit the proliferation ability of HCC cells,and promote the apoptosis process.

关 键 词：细胞凋亡抑制因子1 肝癌 P53 P21 

分 类 号：R735.7[医药卫生—肿瘤]