标记辅助创制富集花青素高油酸的花生种质  

Marker-Assisted Generation of High-oleic Germplasm Accessions Enriched with Anthocyanins in Peanut(Arachis hypogaea L.)

作　　者：李佳伟 马钰聪 李丽 杨鑫雷[1] 崔顺立[1] 刘立峰[1] 王涛 穆国俊[1] LI Jia-wei;MA Yu-cong;LI Li;YANG Xin-lei;CUI Shun-li;LIU Li-feng;WANG Tao;MU Guo-jun(College of Agronomy,Hebei Agricultural University,Baoding 071001;School of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056009;Hebei Yiyuan Ecological Agriculture Technology Co,Ltd,Baoding 074200)

机构地区：[1]河北农业大学农学院,保定071001 [2]河北工程大学园林与生态工程学院,邯郸056009 [3]河北易园生态农业科技有限公司,保定074200

出　　处：《植物遗传资源学报》2022年第4期1037-1045,共9页Journal of Plant Genetic Resources

基　　金：河北省高等学校科学技术研究项目(ZD2019051);河北省重点研发计划项目现代种业科技专项(19226363D);2021年度河北省保定市农业科技园区建设项目(2111N004)。

摘　　要：花生(Arachis hypogaea L.)油酸和花青素含量是花生品质育种的重要目标。本研究以高油酸粉色种皮品系G110为母本和普通油酸紫色种皮品系紫珍珠为父本组配杂交组合。亲本子仁在开花后30 d和45 d的转录组分析结果表明,氧化-还原过程(GO:0055114)和脂肪酸合成过程(GO:0006633)为主要的GO富集代谢通路。差异基因表达结果表明,ahFAD2B(arahy.5913QL)在G110中显著上调表达,而ahFAD2A(arahy.42CZAS)在两个品种中的差异表达水平不显著。FAM tail-1/AlleleX^(a/b)分别确定ahFAD2A和ahFAD2B中的突变型为aabb,HEX tail-2/AlleleY^(a/b)分别确定ahFAD2A和ahFAD2B中的野生型为AABB。利用Kompetitive Allele-Specific PCR(KASP)荧光标记A004807和A004808,在F2筛选出aabb高油酸单株66株。经继代繁育后,在F7得到紫色种皮高油酸种质材料3个,分别为18-B-40、18-B-49和18-B-54,其油酸含量为分别为79.46%、78.77%和78.09%,分别是紫珍珠的1.77倍、0.99倍和0.99倍;油亚比(O/L)分别为14.69、11.89和10.88,分别是紫珍珠的11.58倍、9.37倍和8.58倍;花青素含量分别为30.20 OD/g、28.77 OD/g和29.13 OD/g,分别是G110的23.91倍、22.77倍和23.06倍。本研究获得的富集花青素高油酸花生种质材料对丰富我国高油酸花生种质资源具有重要的现实意义,同时对花生油酸代谢机制的深入研究提供参考。The content of oleic acid and anthocyanin is an important target for quality breeding in peanut(Arachis hypogaea L.).In this study,a high-oleic acid peanut line with pink testa G110 lines(♀)was crossed with a landrace line with purple testa Purple Pearl lines(♂).Kernels of both parent lines on 30 days after flowering and 45 days were sampled for the transcriptomic analysis.The differentially expressed genes(DEGs)enrichments in oxidation-reduction process(GO:0055114)and fatty acid biosynthetic process(GO:0006633)were observed.The gene ahFAD2B(arahy.5913QL)was up-regulated in G110,while differential expression levels of another gene ahFAD2A(arahy.42CZAS)in the two varieties were not significant difference(p>0.05).FAM tail-1/AlleleX^(a/b) identifies mutant genotype aabb in ahFAD2A and ahFAD2B,respectively,and HEX tail-2/AlleleY^(a/b) identifies wild genotype AABB in ahFAD2A and ahFAD2B,respectively.By taking use of kompetitive allele-specific PCR(KASP)markers A004807 and 4004808,66 high-oleic acid plants of genotype aabb in F2 populations were obtained,followed by self-pollination to F7.Of them three superior accessions namely 18-B40,18-B-49 and 18-B-54,with purple testa and high-oleic acid were identified,in which the oleic acid content was 79.52%,78.84%,and 78.02%,1.77 folds,0.99 folds and 0.99 folds higher than that of“Purple Pearl”,respectively.The oleic to linoleic ratio(O/L)was 14.69,11.91,and 10.90,which increased by 11.58 folds,9.37 folds and 8.58 folds compared with that of“Purple Pearl”.The anthocyanin content was 30.87 OD/g,29.16 OD/g,and 14.51OD/g,which increased by 23.91 folds,22.77 folds and 23.06 folds compared with that of G110.Collectively,this study obtained peanut germplasm accessions showing simultaneous enrichments of anthocyanin and high oleic,which might have implications for enriching high oleic peanut germplasms in China and future uncovering the mechanism of peanut oleic acid metabolism.

关 键 词：花生 油酸 花青素 KASP 转录组分析 

分 类 号：S565.2[农业科学—作物学]