多株功能菌种混合培养条件的响应面优化及其生长关系  

Optimization of Mixed Cultivation Conditions with Response Surface Methodology and Growth Relationships of Multiple Functional Strains

作　　者：白红娟 卫燕红 李斌 王海宾 杨官娥[2] 蒋雪琴 胡锦俊 宋雨 BAI Hong-juan;WEI Yan-hong;LI Bin;WANG Hai-bin;YANG Guan-e;JIANG Xue-qin;HU Jin-jun;SONG Yu(Schl.of Environ.&Safety Engin.,N.Uni.of China,Taiyuan 030051;Schl.of Pharm.,Shanxi Med.Uni.,Taiyuan 030001)

机构地区：[1]中北大学环境与安全工程学院,山西太原030051 [2]山西医科大学药学院,山西太原030001

出　　处：《微生物学杂志》2022年第3期27-35,共9页Journal of Microbiology

基　　金：山西省重点研发计划项目(201903D321083);中央引导地方科技发展资金项目(YDZX20201400001443);中北大学研究生科技立项(20201767);中北大学大学生创新创业项目(20210381);。

摘　　要：对3种具有土壤中多种重金属污染修复和病虫害防治功能的光合细菌(Photosynthetic bacteria,PSB,包括Rhodobacter sphaeroides H菌株、Rhodopseudomonas palustris N菌株和Rhodospirillium rubrum M菌株)、枯草芽胞杆菌(Bacillus subtilis,BS)和植物乳酸杆菌(Lactobacillus plantarum,LP)进行混合培养,利用单因素和响应面试验优化培养基配方以及培养条件。确定功能菌混合培养的最佳条件:以光合细菌培养基为基础培养基,外加碳源为10 g/L蔗糖、氮源为1.25 g/L硫酸铵、培养温度为31.5℃、初始pH值为7.1、接种量为接种后的初始培养活菌数1.90×10^(9) cfu/mL、培养时间为28.0 h。通过功能菌的加性模型法、生长动力学以及混合培养的生长关系分析表明,光合细菌、枯草芽胞杆菌和植物乳酸杆菌的生长均存在互惠互利关系。Three kinds of photosynthetic bacteria(PSB for short,including Rhodobacter sphaeroides H strain,Rhodopseudomonas palustris N strain,and Rhodospirillium rubrum M strain),Bacillus subtilis(BS)and Lactobacillus plantarum(LP),which have the functions of repairing multiple heavy metal pollution in the soil and preventing,controlling diseases,and insect pests,were mixed-cultivated.The medium formula and culture conditions were optimized by single-factor and response surface tests.The results showed that the optimal conditions for the mixed-culture of bacteria were as follows:photosynthetic bacteria culture medium as the basic medium with additional 10 g/L sucrose as carbon source,1.25 g/L ammonium sulfate as nitrogen source,culture at 31.5℃,initial pH 7.1,inoculation volume was equal to the initial cultured bacteria content after inoculation 1.90×10^(9) cfu/mL,and culture for 28.0 h.Additive model method,the modified Gompertz equation,and analysis of growth characteristics of mixed-culture of functional bacteria showed all growth relationship of photosynthetic bacteria,Bacillus subtilis,and Lactobacillus plantarum were mutual beneficial.

关 键 词：光合细菌 枯草芽胞杆菌 植物乳酸杆菌 混合培养条件优化 生长关系 

分 类 号：Q93-335[生物学—微生物学]