HPLC-DAD-CAD法同时测定川佛手中5个成分的含量  

Simultaneous determiation of five constituents in citri sarcodactylis fructus by HPLC-DAD-CAD

作　　者：曾利 李雪敏[1] 杨欢[1] 龚倩倩 刘欣 韦佳依 邓放[1] ZENG Li;LI Xuemin;YANG Huan;GONG Qianqian;LIU Xin;WEI Jiayi;DENG Fang(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China)

机构地区：[1]西南特色中药资源国家重点实验室,成都中医药大学药学院,成都611137

出　　处：《环境化学》2022年第6期2143-2145,共3页Environmental Chemistry

基　　金：四川省科技厅重点研发项目(2022YFN0068)。

摘　　要：建立高效液相色谱-二极管阵列检测器-电雾式检测器(HPLC-DAD-CAD)法同时测定川佛手中香叶木苷、橙皮苷、5,7-二甲氧基香豆素、柠檬苦素和诺米林等5个成分含量的方法.研究结果表明,川佛手中香叶木苷、橙皮苷、5,7-二甲氧基香豆素、柠檬苦素和诺米林分别在106.6—533 ng(r=0.9998)、102.4—512 ng(r=0.9999)、103.0—515 ng(r=0.9999)、82.2—411 ng(r=0.9996)、127.6—638 ng(r=0.9994)范围内线性关系良好,平均加样回收率分别为105.04%、107.19%、98.39%、105.01%、94.33%,RSD值分别为1.96%、1.67%、2.72%、2.39%、0.45%.8批样品中橙皮苷、香叶木苷、5,7-二甲氧基香豆素、柠檬苦素和诺米林的百分含量分别为0.026%—0.168%、0.036%—0.081%、0.064%—0.256%、0.016%—0.084%、0.068%—0.116%.建立的HPLC-DAD-CAD测定5个成分含量的方法稳定可靠,重复性好,可为川佛手的质量控制方法研究提供参考.To establish a method for the simultaneous determination of diosmin,hesperidin,5,7-dimethoxycoumarin,limonin and nomilin in Citri Sarcodactylis Fructus by high performance liquid chromatography coupled with diode array and charged aerosol detector(HPLC-DAD-CAD).Results:The concentrations of diosmin,hesperidin,5,7-dimethoxycoumarin,limonin and normilin were 106.6—533 ng(r=0.9998),102.4—512 ng(r=0.9999),103.0—515 ng(r=0.9999)and 82.2—411 ng(r=0.9996),127.6—638 ng(r=0.9994),respectively The average recoveries were 105.04%,107.19%,98.39%,105.01%and 94.33%,respectively.The average recoveries were 105.04%,107.19%,98.39%,105.01%and 94.33%,respectively.RSD values were 1.96%,1.67%,2.72%,2.39%and 0.45%,respectively.The contents of hesperidin,diosmin,5,7-dimethoxycoumarin,limonin and nomilin in 8 batches of samples were 0.026%—0.168%、0.036%—0.081%、0.064%—0.256%、0.016%—0.084%and 0.068%—0.116%,respectively.Conclusion:The established HPLC-DADCAD method is stable,reliable and reproducible,which can provide a reference for the study of quality control methods of Citri Sarcodactylis Fructus.

关 键 词：佛手 高效液相色谱-二极管阵列检测器-电雾式检测器 香叶木苷 橙皮苷 5 7-二甲氧基香 豆素 柠檬苦素 诺米林 

分 类 号：O657.72[理学—分析化学] TS255.7[理学—化学]