基于葡萄有机酸的稀有人参皂苷20(S,R)-Rg3和Rg5的绿色制备  

Green Preparation of Rare Ginsenosides 20(S,R)-Rg3 and Rg5 based on Organic Acids in Grape

作　　者：张浩然 朱小涵 赵迅 李玲 成乐琴[1] ZHANG Haoran;ZHU Xiaohan;ZHAO Xun;LI Ling;CHENG Leqin(School of Chemical and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China;Forensic Center of Changchun Public Security Bureau,Changchun 130000,China;Tonghua Bai'aojinsen Biotechnology Co.,Ltd,Tonghua 134000,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]吉林省长春市公安司法鉴定中心,吉林长春130000 [3]通化百奥金森生物科技有限公司,吉林通化134000

出　　处：《吉林化工学院学报》2022年第3期11-15,50,共6页Journal of Jilin Institute of Chemical Technology

基　　金：吉林省科技厅重点科技研发项目(20180201073YY)。

摘　　要：以人参须根粉为原料,天然葡萄汁作为酸催化剂,自制改良索氏提取器作为提取装置,研究了从人参须根粉直接提取制备富含稀有人参皂苷Rg3、Rg5的人参提取物的方法,并通过单因素实验和正交实验进行了工艺优化.实验结果表明:当乙醇浓度为20%,提取筒温度为60℃,油浴温度为145℃,葡萄汁用量为10 mL,提取转化时间为5 h时,所得的人参皂苷Rg3、Rg5总得率为2.29%.该方法将人参皂苷提取和结构修饰并在一步操作中,固液可直接分离、溶剂用量少,可以快捷、绿色制备高活性稀有人参皂苷.The direct extraction and preparation method of extracting ginseng extract which is rich in Rg3 and Rg5 from ginseng root powder was studied in the paper,and the technical process optimization was carried out by single factor experiment and orthogonal experiment.In the study,ginseng root powder was used as raw material,natural grape juice was used as acid catalyst,self-made improved Soxhlet extractor was used as an extraction device.According to the experimental results,when the concentration of ethanol was 20%,the temperature of extraction tube was 60℃,the temperature of oil bath was 145℃,the dosage of grape juice was 10 mL,and the extraction time was 5 h,the total yield of ginsenoside Rg3 and Rg5 was 2.29%.Ginsenoside’s extraction and structure modification were compressed into one step,and solid-liquid separation could be done directly with less solvent due to the method,so that highly activity ginsenosides could be quickly and environmentally friendly prepared.

关 键 词：葡萄汁 人参须根粉 人参皂苷RG3 人参皂苷Rg5 改良索氏提取器 

分 类 号：O629.21[理学—有机化学]