Adaptive evolutionary expansion of the ribonuclease 6 in Rodentia  

作　　者：Datian LANG Burton KLIM Yun GAO Xiaoping WANG 

机构地区：[1]Agronomy and Life Science Department,Zhaotong University,Zhaotong,China [2]Department of Natural History,Royal Ontario Museum,Toronto,Ontario,Canada [3]State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Science,Kunming,China [4]School of Life Sciences,Yunnan University,Kunming,China [5]State Key Laboratory for Conservation and Utilization of Bio-resource in Yunnan,Yunnan University,Kunming,China [6]Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province,Yunnan University,Kunming,China.

出　　处：《Integrative Zoology》2019年第3期306-317,共12页整合动物学（英文版）

基　　金：the Yunnan Applied Ba­sic Research Project(2017FD148);the State Key Basic Research and Development Plan in Kunming In­stitute of Zoology(GREKF17-02,to DTL);the Na­tional Natural Science Foundation of China(31760619,to XPW).

摘　　要：Ribonuclease 6(RNase6 or RNase K6)is a protein that belongs to a superfamily thought to be the sole verte­brate-specific enzyme known for a wide range of physiological functions,including digestion,cytotoxicity,an­giogenesis,male reproduction and host defense.In our study,51 functional genes and 11 pseudogenes were identified from 27 Rodentia species.Intriguingly,in the 3 main lineages of rodents there were multiple RNas­e6s identified in all species of Ctenohystrica,whereas only a single RNase6 was observed in other Rodentia spe­cies examined except for 2 species in the mouse-related clade.The evolutionary scenario of“birth(gene dupli­cation)and death(gene deactivation)”and gene sorting have been demonstrated in Ctenohystrica.In addition,bursts of positive selection,diversification of isoelectric point and positive net charge have been identified in Ctenohystrica,especially at two key sites that are involved in antimicrobial function.Site Trp30 has undergone positive selection and Ile45 has changed into other residues in Group B and Group C of the Ctenohystrica.Our results demonstrated a complex and intriguing evolutionary pattern of rodent RNase6,and indicated that func­tional modification may have occurred,which establishes an important theoretical foundation for future func­tional assays in rodent RNase6.

关 键 词：gene duplication gene sorting pancreatic ribonuclease 6(RNase6) positive selection RODENTIA 

分 类 号：R73[医药卫生—肿瘤]