不同程度颅脑疾病患者肠道菌群分析  

Intestinal flora in patients with different severities of craniocerebral diseases

作　　者：孙小鹏 何小花 黄利红 张慧婷 任哲 马晓薇 SUN Xiao-peng;HE Xiao-hua;HUANG Li-hong;ZHANG Hui-ting;REN Zhe;MA Xiao-wei(School of Clinical Medical,Ningxia Medical University,Yinchuan,Ningxia 750004,China;不详)

机构地区：[1]宁夏医科大学临床医学院,宁夏银川750004 [2]宁夏医科大学总医院心脑血管病医院重症监护室

出　　处：《中国微生态学杂志》2022年第4期445-450,共6页Chinese Journal of Microecology

基　　金：宁夏医科大学总医院院内资助项目(2015YN005)。

摘　　要：目的检测不同程度颅脑疾病患者肠道菌群分布情况,探讨患者肠道菌群分布与疾病的关系,为该类患者的治疗提供参考。方法通过MiSeq PE2x300 bp高通量测序技术对21例重度颅脑损伤患者(G1组,GCS≤8分)和14例轻度颅脑损伤患者(G2组,GCS>8分)粪便样本中细菌的16S rRNA基因V3-V4可变区进行定性分析,并对两组患者肠道菌群进行生物学分类比较、多样性分析和组间物种差异分析。结果两组患者肠道菌群多样性差异无统计学意义(均P>0.05)。G1组患者肠道厌氧球菌属(Anaerococcus)、埃希菌-志贺菌属(Escherichia-Shigella)、链球菌属(Streptococcus)、肠球菌属(Enterococcus)、棒状杆菌属(Corynebacterium)数量高于G2组(均P<0.05)。结论颅脑疾病患者肠道菌群紊乱,其肠道菌群丰度及多样性显著下降,有害菌数量升高,有益菌数量下降。Objective To investigate the distribution of intestinal flora in patients with different severities of craniocerebral diseases,and analyze the relationship between the distribution of intestinal flora and the diseases,so as to provide a reference for the treatment.Methods The V3-V4 variable regions of 16 S rRNA genes in fecal samples of 21 patients with severe craniocerebral injury(G1 group,GCS≤8)and 14 patients with mild craniocerebral injury(G2 group,GCS>8)were analyzed by using MiSeq PE2 x300 bp high-throughput sequencing technique.The biological classification,diversity and species of intestinal flora were analyzed and compared between groups.Results There was no significant difference in the diversity of intestinal flora between the two groups(P>0.05).The abundances of intestinal Anaerococcus,Escherichia-Shigella,Streptococcus,Enterococcus and Corynebacterium in G1 group were higher than those in G2 group(all P<0.05).Conclusion The intestinal flora of patients with severe craniocerebral disease are obviously disordered;The abundance and diversity of intestinal flora decreased significantly,the proportion of harmful bacteria increased and that of beneficial bacteria decreased significantly.

关 键 词：颅脑疾病重症患者 GCS评分 肠道菌群 多样性 16S rRNA 

分 类 号：R743.3[医药卫生—神经病学与精神病学]