果汁中乙醇质量浓度的抗干扰检测方法  

Anti-interference detection method of ethanol concentrationin fruit juices

作　　者：王丙莲 马耀宏 公维丽 杨艳 郑岚 刘庆艾 孟庆军 WANG Bing-lian;MA Yao-hong;GONG Wei-li;YANG Yan;ZHENG Lan;LIU Qing-ai;MENG Qing-jun(Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省科学院生物研究所,山东济南250103

出　　处：《山东科学》2022年第4期84-89,共6页Shandong Science

基　　金：山东省自然科学基金(ZR2019BC072);山东省重点研发计划(重大科技创新工程)(2020CXGC010602);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2020KJC-ZD15)。

摘　　要：针对生物传感器法测定果汁中乙醇质量浓度易受电化学活性物质干扰的问题,通过制备乙醇辅助酶电极,使工作酶电极和辅助酶电极协同工作,构建了一种新型的乙醇检测系统,实现了乙醇含量快速准确的检测。结果表明,该检测系统用于果汁乙醇质量浓度检测时,稳定性好、结果准确,抗干扰能力强。重复测定的相对标准偏差为0.80%;加标回收率为99.10%~101.10%;葡萄糖、蔗糖、柠檬酸、Ca^(2+)、过氧化氢酶以及电化学活性物质维生素C等干扰物存在的情况下,乙醇测定结果不受影响;与国标法、气相色谱法相比较,差异不明显(P>0.05)。该方法操作简单、成本低廉且准确度高,能够满足低成本要求的实际生产要求。To eliminate the influence of electrochemically active compounds on ethanol determination in fruit juices via biosensor,an ethanol-assisted enzyme electrode was prepared and a novel alcohol biological detection system was constructed using the synergy of working and auxiliary enzyme electrodes.The anti-interference property of the system was tested and the anti-interference coefficient was fitted.Thereafter,the calculation formula of ethanol concentration was deduced to achieve the rapid and accurate detection of ethanol concentration.The results showed that the anti-interference performance of the detection was stable with good stability,accurate results,and strong ability.When the method was applied to determine the ethanol concentration in fruit juices,the relativity standard division was 0.80%and the recoveries were 99.10%to 101.10%.Glucose,sucrose,citric acid,Ca^(2+),catalase,and vatamine C in the fruit juices had no effect on ethanol detection.T-test analysis showed that there was no significant difference between this method and the national standard and gas chromatography(P>0.05).The method is simple in operation,low in cost and high in accuracy,which can meet the production requirements of low cost.

关 键 词：果汁 乙醇检测 辅助酶电极 抗干扰 

分 类 号：TS275.5[轻工技术与工程—农产品加工及贮藏工程]