机械敏感离子通道Piezo1在糖尿病大鼠牙移动过程中的表达和功能研究  

Expression and function of mechanosensitive ion channel Piezo1 during tooth movement in diabetic rats

作　　者：关禹哲 蒋玉坤 吴祖平 李天成 胡芝爱 邹淑娟[1] GUAN Yuzhe;JIANG Yukun;WU Zuping;LI Tiancheng;HU Zhiai;ZOU Shujuan(State KeyLaboratoryofOralDiseases,National Center for Clinical Medicine of Oral Diseases,Department of Orthodontic,West China Hospital of Stomatology Sichuan University,Chengdu 610041,China)

机构地区：[1]口腔疾病研究国家重点实验,室国家口腔疾病临床医学研究中心,四川大学华西口腔医院正畸科,四川成都610041

出　　处：《口腔医学》2022年第6期487-493,共7页Stomatology

基　　金：国家自然科学基金(82071150);四川省卫健委普及应用项目(20PJ087)。

摘　　要：目的研究糖尿病大鼠正畸牙齿移动中张力侧牙周组织内Piezo1的表达变化,探索其在张力侧牙周组织改建中的作用。方法选取60只健康雄性Wistar大鼠为研究对象,分成对照组以及糖尿病组,以双侧切牙为支抗施加40 g拉伸力近中移动左侧第一磨牙,分别在0、7、14 d处死大鼠后获得左上后牙区牙槽骨块。HE染色检测组织形态变化,免疫组化及荧光染色检测张力侧Piezo1、Osterix以及Runx2的表达水平,Micro-CT检测张力侧骨组织相关参数指标。结果正畸牙移动激活了张力侧牙周膜中Piezo1的表达,糖尿病大鼠Piezo1以及成骨分化关键转录因子Osterix和Runx2的表达显著低于健康大鼠。结论糖尿病显著抑制了正畸牙移动过程中张力侧牙周膜中Piezo1的表达,降低了张力侧成骨活性,抑制正畸牙移动张力侧牙周组织的改建。Objective To study the expression of Piezo1 on the tension side of periodontal tissues during orthodontic tooth movement in diabetic rats and explore its role in the remodeling of periodontal tissues.Method Sixty male Wistar rats were divided into the control group and the diabetic group.With bilateral incisors as anchors,40 g stretching force was applied to move the left first molar forward.Rats were sacrificed at 0 day,the 7;day,and the 14;day,and the posterior part of the left maxillary was obtained.HE staining,immunohistochemistry and immunofluorescence were used to examine histological changes and expression levels of Piezo1,Osterix and Runx2.Micro-CT was used to detect parameters related to bone tissues on the tension side.Results During orthodontic tooth movement,the expression of Piezo1 was upregulated by the orthodontic force in the control group on the tension side.The expression level of Piezo1,and osteogenic markers Osterix and Runx2 was significantly decreased in the diabetic group compared to the control group.Conclusion The expression of the Piezo1 and osteogenic activity on the tension side are significantly inhibited in the diabetic group during orthodontic movement.Diabetes could prohibit tissue remodeling on the tension side during orthodontic tooth movement.

关 键 词：正畸 牙移动 Piezo1 糖尿病 

分 类 号：R78305[医药卫生—口腔医学]