Anti-angiogenesis and anti-inflammatory effects of Moringa oleifera leaf extract in the early stages of streptozotocin-induced diabetic nephropathy in rats  

作　　者：Ruttiya Thongrung Laddawan Senggunprai Wiphawi Hipkaeo Panot Tangsucharit Patchareewan Pannangpetch 

机构地区：[1]Department of Pharmacology,Faculty of Medicine,Khon Kaen University,Khon Kaen,40002,Thailand [2]Cardiovascular Research Group,Khon Kaen University,Khon Kaen,40002,Thailand [3]Department of Anatomy,Electron Microscopy Unit,Faculty of Medicine,Khon Kaen University,Khon Kaen,40002,Thailand

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第7期290-299,共10页亚太热带生物医学杂志（英文版）

基　　金：supported by the Cardiovascular Research Group,Khon Kaen University and the Faculty of Medicine,Khon Kaen University,Thailand(grant number IN63355).

摘　　要：Objective:To investigate the effect of Moringa oleifera leaf extract on angiogenesis and inflammatory process in a rat model of streptozotocin-induced diabetic nephropathy.Methods:Four weeks after a single injection of 50 mg/kg streptozotocin,rats were treated with 100 or 200 mg/kg/day Moringa oleifera leaf extract,1 mg/kg/day dapagliflozin,or a combination of Moringa oleifera leaf extract and dapagliflozin for further eight weeks.Renal function,kidney histology,and gene expression were evaluated at the end of the experiment.Results:Renal function of diabetic rats was significantly impaired as evidenced by increased blood urea nitrogen,albuminuria,24-h proteinuria,and high creatinine clearance which indicated glomerular hyperfiltration.In addition,diabetic rats showed an increase in gene expressions of vascular endothelial growth factor-A(VEGF-A),angiopoietin-2(Ang2),the Ang2/Ang1 ratio,tumor necrosis factor-α,interleukin-1βand monocyte chemoattractant protein-1.Immunohistochemical staining demonstrated a significant increase in the density of glycoprotein CD34.Moringa oleifera leaf extract markedly improved all renal dysfunction markers and modulated the upregulated expression of angiogenic factors and inflammatory genes.Conclusions:Moringa oleifera leaf extract could suppress abnormal angiogenesis and inflammatory processes possibly by downregulating gene expression of angiogenesis factors and proinflammatory cytokines.

关 键 词：Moringa oleifera leaf extract Diabetic nephropathy ANGIOGENESIS ANGIOPOIETIN VEGF-A Inflammation 

分 类 号：R285.5[医药卫生—中药学]