人牙周膜成纤维细胞来源外泌体对大鼠延期牙再植术后牙根吸收的调控作用  

Effects of human periodontal ligament fibroblast-derived exosomes on reducing rat root resorption after delayed tooth replantation

作　　者：尹悦 陶硕 张旗[1] YIN Yue;TAO Shuo;ZHANG Qi(Department of Endodontics,Schoolof Stomatology,Tongji Universily,Shanghai Engineering Research Center of Tooth Restoralion and Regeneralion,Shanghai 200072,China)

机构地区：[1]同济大学口腔医学院牙体牙髓病学教研室,上海牙组织再生与修复工程技术研究中心,上海200072

出　　处：《上海口腔医学》2022年第3期232-236,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81870760、81570966);中央高校基本科研业务费专项资金(22120190217)。

摘　　要：目的 :探讨健康的人牙周膜成纤维细胞(hPDLFs)来源外泌体对大鼠延期牙再植术后牙根吸收的作用及其可能机制。方法:分离提取hPDLFs来源的外泌体并鉴定。选择30只6周龄雄性SD大鼠,随机分为对照组和外泌体组,建立上颌第一磨牙延期牙再植术模型,牙脱位30 min后植回牙槽窝。对照组牙周膜局部注射40μL汉克斯平衡盐溶液(HBSS),实验组牙周膜局部注射40μL含外泌体的HBSS。术后1、2、4周收样,苏木精-伊红(H-E)染色观察牙根表面吸收陷窝,抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞数量,免疫组织化学染色观察牙周膜内骨保护素(OPG)的表达。采用SPSS 17.0软件包对数据进行统计学处理。结果:鉴定表明提取的细胞外囊泡为外泌体。与对照组相比,hPDLFs来源的外泌体减少了延期牙再植术后牙根吸收陷窝的数量,降低TRAP阳性破骨细胞的表达(P<0.05),促进牙周膜内OPG的表达(P<0.05)。结论:延期牙再植术后,hPDLFs来源的外泌体减少了破骨细胞的数量,促进牙周膜内OPG表达,减少了再植术后的牙根吸收。PURPOSE: To explore the effect of healthy human periodontal ligament fibroblasts(hPDLFs)-derived exosomes on tooth resorption after delayed tooth replantation in rats and its possible mechanism. METHODS: The exosomes derived from hPDLFs were isolated and identified in thirty six-week-old SD rats and randomly divided into control group and exosome group. The right maxillary first molar was extracted to establish a delayed tooth replantation model. The dislo-cated teeth were implanted back into the alveolar fossa after 30 minutes. 40 μL Hanks’ balanced salt solution(HBSS) were injected into the periodontal tissue, and the experimental group was injected with 40 μL HBSS containing exosomes.The rats were sacrificed at 1, 2, and 4 weeks. Hematoxylin-eosin(H-E) staining was used to observe tooth resorption. Tar-trate-resistant acid phosphatase(TRAP) staining was used to observe the number of osteoclasts. The expression of osteopro-tegerin(OPG) in periodontal ligament was detected by immunohistochemical staining. The differences in distribution of each genotype were analyzed with SPSS 17.0 software package. RESULTS: The identification experiment showed that extracellular vesicles were exosomes. hPDLFs-derived exosomes inhibited root resorption after delayed tooth replantation, re-duced the expression of TRAP-positive osteoclasts(P<0.05), and promoted expression of OPG in periodontal ligament(P<0.05). CONCLUSIONS: After delayed tooth replantation, PDLFs-derived exosomes reduce the number of osteoclasts,promote OPG expression in the periodontal ligament, and reduce tooth root resorption after replantation.

关 键 词：外泌体 牙根吸收 延期牙再植 骨保护素 

分 类 号：R78[医药卫生—口腔医学]