炎症微环境对人牙周膜成纤维细胞增殖与成骨分化的影响  

The effect of inflammation on proliferation and osteogenic differentiation of periodontal ligament cells

作　　者：董家辰[1] 束蓉[1] DONG Jiachen;SHU Rong(Depurment of Periodonology,Shonghai Ninth People’s Hospial,Shanghui Jioo Tong Universiy School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院牙周病科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2022年第3期243-247,共5页Shanghai Journal of Stomatology

基　　金：上海交通大学医学院附属第九人民医院交叉基金资助(JYJC201904)。

摘　　要：目的 :观察脂多糖模拟的炎症微环境对体外培养的人牙周膜成纤维细胞(periodontal ligament cells, PDLCs)增殖与成骨分化的影响。方法:体外培养鉴定人牙周膜成纤维细胞,分别用浓度为0、0.1、10μg/mL脂多糖作用于细胞,采用MTT法观察脂多糖对PDLCs增殖的影响,运用实时定量PCR和Western印迹法检测脂多糖模拟的炎症微环境对PDLCs成骨分化的影响。采用SPSS 13.0软件包对数据进行统计学分析。结果:0.1μg/mL脂多糖促进PDLCs增殖,增强成骨标志物mRNA和蛋白的表达;10μg/mL脂多糖可抑制PDLCs的增殖及碱性磷酸酶、RUNX2、Ⅰ型胶原、骨形态发生蛋白2的表达,其结果有统计学意义。结论:高浓度脂多糖(10μg/mL)模拟的炎症微环境对PDLCs增殖及成骨分化有抑制作用,低浓度脂多糖(0.1μg/mL)对PDLCs的增殖和成骨分化有促进作用。PURPOSE: To investigate the effects of inflammatory microenvironment on proliferation and osteogenic differ-entiation of periodontal ligament cells(PDLCs) in vitro. METHODS: Human PDLCs were isolated and characterized. MTT was used to investigate the proliferation rate of PDLCs under different concentration of lipopolysaccharide(LPS). The PDLCs’ osteogenic differentiation was investigated using real-time PCR and Western blot. The date were statistically ana-lyzed with SPSS 13.0 software package. RESULTS: Treatment with 0.1 μg/mL LPS increased proliferation of PDLCs and enhanced the expression of osteogenic gene and protein. The proliferation of PDLCs and expression of alkaline phos-phatase(ALP), RUNX2, Collagen-I, BMP2 were significantly decreased by 10 μg/mL LPS. CONCLUSIONS: The inflammatory microenvironment(10 μg/mL LPS) inhibits the proliferation and osteogenic differentiation of human PDLCs.

关 键 词：人牙周膜成纤维细胞 炎症微环境 脂多糖 成骨分化 

分 类 号：R781.4[医药卫生—口腔医学]