细胞色素P450酶2A6基因多态性与123例汉族吸烟人群牙周炎及炎性细胞因子表达水平的关系  

Relationship between CYP2A6 gene polymorphism and periodontitis, expression of inflammatory cytokines in 123 Han smokers with periodontitis

作　　者：向映辉[1] 王占刚 李丽娟[2] 王利娟[1] 汤晨 XIANG Ying-hui;WANG Zhan-gang;LI Li-juan;WANG Li-juan;TANG Chen(Department of Endodontics,Changzhou Stomatological Hospital.Changzhou 213003 Jiangsu Prouince;Department of Stomalology,Scond Afiliaed Hospital of Guangehou Medical Universily Guanghou 510260,Guangdong Province China)

机构地区：[1]常州市口腔医院牙体牙髓病科,江苏常州213003 [2]广州医科大学附属第二医院口腔科,广东广州510260

出　　处：《上海口腔医学》2022年第3期286-289,共4页Shanghai Journal of Stomatology

基　　金：常州市卫生健康委科技项目(WZ201914)。

摘　　要：目的:检测细胞色素P450酶2A6(CYP2A6)基因多态性与汉族吸烟人群牙周炎及炎性细胞因子表达水平的关系。方法:收集2018年10月—2019年10月常州市口腔医院收治的123例吸烟牙周炎患者为实验组,同期125例非吸烟牙周炎患者为对照组。收集患者一般资料,包括年龄、性别、体质量指数(BMI)、咀嚼、刷牙习惯和磨牙情况;检测菌斑指数(PLI)、牙龈出血指数(BI)、牙周探诊深度(PD)和附着丧失(AL)。采用PCR扩增CYP2A6;酶联免疫吸附试验(ELISA)检测龈沟液中炎性细胞因子白介素(IL)17、IL-1、IL-6、IL-23和肿瘤坏死因子α(TNF-α)水平。采用SPSS25.0软件包对数据进行统计学分析。结果:2组性别,PLI,龈沟液中IL-17、IL-1、IL-6、IL-23、TNF-α水平存在显著差异(P<0.05)。对所有样品进行PCR扩增,未扩增出的23例判定为CYP2A6缺失型(CYP2A6del),其中,实验组5例、对照组18例;扩增出的225例判定为CYP2A6野生型(CYP2A6wt),其中,实验组118例、对照组107例。2组CYP2A6基因型存在显著差异(P<0.05)。实验组不同CYP2A6基因型IL-1水平、PLI存在显著差异(P<0.05);对照组不同CYP2A6基因型IL-17水平、PLI存在显著差异(P<0.05)。结论:CYP2A6基因型在汉族人群牙周炎患者是否吸烟中存在差异,但与炎性细胞因子水平的关系尚不明确。PURPOSE: To investigate the relationship between polymorphism of cytochrome P450, family 2, subfamily A,polypeptide 6(CYP2A6) and periodontitis, the expression of inflammatory cytokines in 123 Han smokers. METHODS:From October 2018 to October 2019, a total of 123 smokers with periodontitis were selected as the experimental group,and 125 non-smokers as the control group. The general data of the patients were collected, including age, gender, body mass index(BMI), chewing and brushing habits, as well as molar condition;plaque index(PLI), gingival bleeding index(BI), periodontal probing depth(PD) and attachment loss(AL) were detected. CYP2A6 was amplified by PCR. The level of interleukin(IL)-17, IL-1, IL-6, IL-23 and tumor necrosis factor-α(TNF-α) in GCF was detected by enzyme-linked immunosorbent assay(ELISA). SPSS 25.0 software package was used for statistical analysis of the data. RESULTS: There was significant difference in gender, PLI, IL-17, IL-1, IL-6, IL-23, TNF-α level in GCF between the two groups(P<0.05).All samples were amplified by PCR. Among them, 23 were not amplified, which were identified as CYP2A6 deletion type(CYP2A6del), includ ing 5 in the experimental group and 18 in the control group;225 were amplified and identified as CYP2A6 wild type(CYP2A6wt), including 118 in the experimental group and 107 in the control group. There was significant difference in CYP2A6 genotype between the two groups(P<0.05). In the experimental group, the level of IL-1 and PLI of different CYP2A6 genotypes was significantly different(P<0.05);and in the control group, the level of IL-17 and PLI of different CYP2A6 genotypes was also significantly different(P<0.05). CONCLUSIONS: There are differences in CYP2A6genotype between smokers and non-smokers in Han population with periodontitis, but the relationship between CYP2A6genotype and inflammatory cytokines is not clear.

关 键 词：细胞色素P450酶2A6 基因多态性 汉族 吸烟人群 牙周炎 炎性细胞因子 

分 类 号：R781.4[医药卫生—口腔医学]