CysLTR1通路调节铝负荷肝细胞氧化应激及凋亡机制  

CysLTR1 pathway regulates oxidative stress and apoptosis mechanism of aluminum-loaded hepatocytes

作　　者：胡聪俐 杨俊卿 李欢 杨璐 杨洋 王红 HU Cong-li;YANG Jun-qing;LI Huan;WANG Hong(Department of Pharmacy,Wenzhou Hospital of Traditional Chinese Medicine(Wenzhou,Zhejiang,32500),China;Department of Pharmacology,School of Pharmacy,Chongqing Medical University)

机构地区：[1]温州市中医院药剂科,浙江温州325000 [2]重庆医科大学药学院药理学教研室

出　　处：《中西医结合肝病杂志》2022年第7期614-616,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：重庆市基础与前沿研究(No.cstc2013jcyjA10004)。

摘　　要：目的:研究CysLTR1通路调节铝负荷肝细胞氧化应激及凋亡的作用机制。方法:HL-7702细胞用RPMI-1640培养,分为正常对照组、铝负荷组、MK-571+铝组,分别给予相应药物干预后,用MTT法检测细胞活力,确定CysLTR1抑制剂(MK-571)给药浓度。采用细胞流式技术检测各组细胞凋亡的情况,运用试剂盒检测各组细胞中的ALT、AST水平,SOD和MDA含量。运用WB技术测定各组细胞中Caspase3、Caspase9、Bcl-2和BAX的蛋白表达。结果:给予MK-571(3nmol/L)可以提高铝负荷L02细胞生存率(P<0.01),并降低铝负荷L02细胞凋亡指数(P<0.01),降低铝负荷细胞中ALT和AST水平(P<0.01),减少MDA的含量(P<0.01),同时提高SOD的活力(P<0.01)。MK-571还可以下调铝负荷细胞中Caspase3、Caspase9和BAX蛋白的表达(P<0.05和P<0.01),上调Bcl-2蛋白的表达(P<0.05)。结论:CysLTR1通路调节铝负荷肝细胞氧化应激,影响细胞凋亡机制,保护铝负荷肝细胞凋亡。Objective:To investigate the mechanism of the CysLTR1 signal pathway on oxidative stress and apoptosis of aluminum-loaded hepatocytes.Methods:HL-7702 cells were cultured in RPMI-1640 medium and divided into normal control group,aluminum loaded group and MK-571 group.Each group was given corresponding drug.Cell viability was detected by MTT assay to determine the concentration of MK-571.The apoptosis rate of each group was measured by flow cytometry(FCM),and the activities of ALT,AST,SOD,and MDA in the cells of each group were measured by the kits.The expressions of Caspase3,Caspase9,Bcl-2,and BAX proteins were measured by western blot assay.Results:The CysLTR1 inhibitor(MK-571;3 nM,36 hours)could validly enhance the survival rate(P<0.01),decreased the rate of apoptosis in aluminum-loaded L02 cells(P<0.01).The content of ALT,AST,and MDA were significantly decreased by treating with MK-571(P<0.01),and the activity of SOD was increased(P<0.01).Meanwhile,the expression of Caspase3,Caspase9,and BAX proteins were down-regulated significantly(P<0.05,P<0.01),and the level of Bcl-2 protein was up-regulated(P<0.05)treated with MK-571 in aluminum-loaded L02 cells.Conclusion:CysLTR1 pathway regulates oxidative stress,affects the apoptosis mechanism,and protects aluminum-loaded hepatocytes from apoptosis.

关 键 词：肝细胞 氧化应激 凋亡CysLTR1通路 铝负荷 

分 类 号：R575[医药卫生—消化系统]