木鳖子调控circRNA_002178表达抑制肝癌细胞增殖、迁移及侵袭的作用机制研究  

Momordica cochinchinensis regulates the expression of circRNA_002178 to inhibit the proliferation,migration,and invasion of liver cancer cells

作　　者：欧畅 张建军 钟欢 王媛 何艳霞 宋益青 OU Chang;ZHANG Jian-jun;ZHONG Huan;SONG Yi-qing(Department of oncology,Yongchuan Hospital of traditional Chinese medicine(Chongqing,402160),China)

机构地区：[1]重庆市永川区中医院肿瘤科,重庆402160

出　　处：《中西医结合肝病杂志》2022年第7期622-625,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨木鳖子(MC)对肝癌细胞增殖、迁移、侵袭的影响及其对环状RNA_002178(circRNA_002178)的调控作用。方法:体外培养人肝癌细胞MHCC97-H,不同剂量的MC处理细胞,分别将si-circRNA_002178、pcDNA-circRNA_002178转染至MHCC97-H细胞;CCK-8、Transwell小室实验分别检测增殖、迁移及侵袭;qRT-PCR法检测circRNA_002178的表达量;Western blot法检测MMP-2、MMP-9与PI3K/AKT信号通路相关蛋白p-PI3K、p-Akt的表达量。结果:MC可明显降低细胞存活率与MMP-2、MMP-9、p-PI3K、p-Akt蛋白水平及circRNA_002178的表达水平(P<0.05),减少迁移及侵袭细胞数(P<0.05);转染si-circRNA_002178可明显降低细胞存活率及MMP-2、MMP-9、p-PI3K、p-Akt蛋白水平,减少迁移及侵袭细胞数(P<0.05);转染pcDNA-circRNA_002178可明显降低木鳖子对MHCC97-H细胞增殖、迁移、侵袭及PI3K/AKT信号通路的抑制作用。结论:木鳖子可通过下调circRNA_002178的表达从而抑制肝癌细胞增殖、迁移及侵袭,其作用机制可能与抑制PI3K/AKT信号通路的活化有关。Objective:To explore the effect of MC on the proliferation,migration,and invasion of hepatoma cells and its regulation on circRNA_002178.Methods:Human hepatoma cells MHCC97-H were cultured in vitro,cells were treated with different doses of MC,and si-circRNA_002178 and pcDNA-circRNA_002178 were transfected into MHCC97-H cells,respectively.CCK-8 and transwell chamber experiments were used to detect proliferation,migration,and invasion,respectively.The expression of circRNA_002178 was detected by qRT-PCR method.Western blot was used to detect the expression levels of MMP-2,MMP-9,and PI3K/AKT signaling pathway related proteins p-PI3K and p-Akt.Results:MC could significantly reduce the cell survival rate and the protein levels of MMP-2,MMP-9,p-PI3K,p-Akt and the expression level of circRNA_002178(P<0.05),and reduce the number of migrating and invasive cells(P<0.05).Transfection of si-circRNA_002178 could significantly reduce cell survival rate and protein levels of MMP-2,MMP-9,p-PI3K,and p-Akt,and reduce the number of migrating and invading cells(P<0.05).Transfection of pcDNA-circRNA_002178 could significantly reduce the inhibitory effect of momordica cochinchinensis on the proliferation,migration,invasion and PI3K/AKT signaling pathway of MHCC97-H cells.Conclusion:Momordica cochinchinensis could inhibit the proliferation,migration,and invasion of liver cancer cells by down-regulating the expression of circRNA_002178,and its mechanism may be related to the inhibition of the activation of PI3K/AKT signaling pathway.

关 键 词：肝癌 木鳖子 circRNA_002178 增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]