咪喹莫特致不同免疫状态小鼠银屑病模型比较研究  

Comparative study on imiquimod-induced psoriasis models in mice with different immune states

作　　者：赵亚 吴亚运 刘丽娟 赵瑞芝 ZHAO Ya;WU Yayun;LIU Lijuan;ZHAO Ruizhi(the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;State Key Laboratory of Dampaness Syndrome of Chinese Medicine,Guangzhou 510006;Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome,Guangzhou 510006)

机构地区：[1]广州中医药大学第二附属医院,广州510006 [2]省部共建中医湿证重点实验室,广州510006 [3]广东省中医症候研究重点实验室,广州510006

出　　处：《中国比较医学杂志》2022年第6期41-47,共7页Chinese Journal of Comparative Medicine

基　　金：广东省中医症候研究重点实验室项目(ZH2019ZZ05);省部共建中医湿证重点实验室项目(SZ2021ZZ51)。

摘　　要：目的通过建立不同免疫状态小鼠银屑病模型探讨不同免疫细胞及非免疫因素对银屑病发病的影响。方法Balb/c小鼠、裸鼠、NOG小鼠各20只,分别分为空白组和模型组,每组10只。模型组均采用咪喹莫特涂抹小鼠背部皮肤诱导类银屑病模型,末次造模后取材,检测皮肤、脾病理组织,皮肤炎症因子,以及皮肤和脾能量代谢因子等指标。结果与正常组相比,3种模型动物小鼠皮肤表观状态及病理组织均表现出银屑病样病变,炎症因子(IL6、IL17、IL22、IL23)水平均显著升高,脾均出现肿大,脾病理切片显示Balb/c小鼠,裸鼠白髓区增大,淋巴细胞显著增多,皮肤及脾中的Na^(+)K^(+)-ATP酶和Ca^(2+)Mg^(2+)-ATP酶活性均下降,3种模型成模的严重程度为Balb/c小鼠>裸鼠>NOG鼠。结论多种免疫细胞活化特别是T细胞活化在银屑病发病中具有关键作用,但免疫细胞严重缺失的动物仍能诱发银屑病,其中非免疫因素ATP-ATP酶活性下降可能也是银屑病发病的诱导因素之一。Objective To explore the effects of different immune cells and nonimmune factors on the pathogenesis of psoriasis.Methods Balb/c mice,nude mice and NOG mice were each divided into a blank group and model group,with 10 mice in each group.In the model groups,imiquimod was applied to the back skin of the mice to induce a psoriasislike model,and samples of the skin and spleen were taken after the last application of imiquimod.The pathological changes to the skin and spleen induced by imiquimod were observed,and skin inflammatory factors and energy metabolic factors of the skin and spleen were detected.Results Compared with the normal group,the skin appearance and pathological tissue of the model group in all three mouse types showed psoriasis-like lesions,and the levels of inflammatory factors(IL6,IL17,IL22 and IL23)were significantly higher.In the model groups,the spleen was swollen for all three mouse types,and the pathological spleen section exhibited a larger white pulp area and higher amount of lymphocytes compared with the blank groups in the Balb/c mice and nude mice.The activities of Na^(+)K^(+)-ATPase and Ca^(2+)Mg^(2+)-ATPase in the skin and spleen were lower in the model groups.The relative severity of the three models was as follows:Balb/c mice>nude mice>NOG mice.Conclusions Immune cell activation,especially T-cell activation,plays a key role in the pathogenesis of psoriasis,but psoriasis can still be induced in animals with a severe immune deficiency.A decrease in ATPase activity,which is a non-immune factor,may also be an inducing factor of psoriasis.

关 键 词：银屑病 动物模型 免疫细胞 ATP酶 

分 类 号：R-33[医药卫生]