乳腺癌组织中DNA修复蛋白Ku80的表达及其生物学意义  

The Expression and Biological Significance of Ku80 in Breast Cancer

作　　者：胡李珩 黄前川 洪伟 HU Liheng;HUANG Qianchuan;HONG Wei(Tongcheng County People’s Hospital,Xianning City,Xianning 437400,China;Wuhan Zhongtong Lanbo Clinical Testing Office,Wuhan 430000,China)

机构地区：[1]湖北省咸宁市通城县人民医院,湖北咸宁437400 [2]武汉中核中同蓝博医学检验实验室,湖北武汉430000

出　　处：《标记免疫分析与临床》2022年第4期677-681,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨乳腺癌组织中Ku80蛋白的表达及其生物学意义。方法选取武汉中核中核中同蓝博医学检验实验室2010年1月至2019年12月确诊的80例原发性乳腺癌患者的手术切除标本,采用免疫组织化学分析原发性乳腺癌组织Ku80的表达与细胞增殖相关活性蛋白(Ki67)、基质金属蛋白酶2(MMP-2)、人表皮生长因子受体2(HER2)、P53的相关性;培养人乳腺癌细胞BT-474,分为siRNA-Ku80组、siRNA-阴性对照组和空白对照组,应用Western Blot、Transwell法和MTT法分别检测各组Ki67、MMP-2、P53和HER2水平、细胞侵袭能力和增殖情况。结果乳腺癌组织中Ku80与HER-2、Ki67和MMP-2的表达呈正相关(P<0.05),与P53的表达无明显相关性(P>0.05);Western Blot结果显示siRNA-Ku80组乳腺癌细胞HER2,Ki67和MMP-2蛋白的表达下降,与对照组和空白对照组差异有统计学意义(P<0.05),P53蛋白的表达水平无明显变化(P>0.05);Transwell结果显示siRNA-Ku80组侵袭细胞计数明显减少,与对照组和空白对照组差异有统计学意义(P<0.05);MTT结果显示siRNA-Ku80对乳腺癌细胞BT-474生长有抑制作用,在24h、48h和72h的抑制率分别为9.83%、39.71%和51.20%,与空白组和siRNA对照组比较差异有统计学意义(P<0.05)。结论乳腺癌组织中Ku80的表达与HER-2、Ki67和MMP-2的表达呈正相关;RNA干扰抑制Ku80后,乳腺癌细胞HER-2、Ki67和MMP-2的表达减少,乳腺癌细胞的增殖力和侵袭力降低;提示Ku80的异常表达可能与乳腺癌发生相关。Objective To investigate the expression of Ku80 protein in breast cancer and its biological significance.Methods Specimens of 80 patients with primary breast cancer diagnosed in Wuhan Zhongtong Lanbo Medical Laboratory from January,2010 to December,2019 were analyzed by immunohistochemistry.Human breast cancer cells BT-474 were cultured and all cells were divided into the siRNA-Ku80 group,siRNA-negative control group and blank control group.The expression of HER-2,Ki67,MMP-2 and P53 were detected by Western Blot,while cell proliferation,migration and invasion were detected by MTT and trans-well chamber assays.Results Ku80 was positively correlated with expressions of HER-2,Ki67 and MMP-2 in breast cancer tissues(P<0.05),and Ku80 had no significant correlation with the expression of P53(P>0.05);When Ku80 gene was silenced,levels of HER2,Ki67 and MMP-2 proteins in breast cancer cells decreased significantly(P<0.05),but no effect on the expression P53 protein was observed(P>0.05);Trans-well test results showed that compared with the siRNA-negative control group and blank control group,the number of invasive cells in the siRNA-Ku80 group were significantly decreased(P<0.05);MTT assay indicated that siRNA-Ku80 inhibited BT-474 cells growth with the inhibition rates of 9.83%at 24h,39.71%at 48h,and 51.20%at 72h,which were significantly higher than those in the siRNA-negative control group and blank control group(P<0.05).Conclusion The expression of Ku80 in breast cancer tissues is positively correlated with expressions of HER-2,Ki67 and MMP-2.Silencing of Ku80 gene could inhibit proliferation and invasion of BT-474 cells,therefore,Ku80 abnormal expression may be related to breast cancer.

关 键 词：KU80 乳腺癌 细胞增殖 侵袭力 转移 

分 类 号：R737.9[医药卫生—肿瘤]