都匀毛尖本地种茶树代谢组研究  

Study on Metabolomics of Native Tea Plant Named Duyun Maojian

作　　者：裴会敏 陈志 张龙芬 文狄 王芬 PEI Huimin;CHEN Zhi;ZHANG Longfen;WEN Di;WANG Fen(The Department of Life Science and Agriculture,Qiannan Normal University for Nationalities,Duyun Guizhou 558000,China;Agriculture and Rural Affairs Bureau of Changshun,Changshun Guizhou 550700,China)

机构地区：[1]黔南民族师范学院生物科学与农学院,贵州都匀558000 [2]长顺县农业农村局,贵州长顺550700

出　　处：《种子》2022年第6期27-34,共8页Seed

基　　金：国家自然基金项目(31900486);贵州省植物学重点支持学科(黔学位合字ZDXK[2016]23号);贵州省科技计划项目(黔科合基础[2019]1298);贵州省教育厅项目([2015]68、QNYSKYTD 2018006);黔南民族师范学院科研创新团队(Qnsyk 201605、QNYSKYTD 2018011);黔南民族师范学院项目(2019 xjg 0303、2018 xjg 0520、QNSY 2018 ZJ 006);黔南民族师范学院生物学一流培育学科(QNYSXXK 2018005)。

摘　　要：为了研究都匀毛尖茶本地种茶树不同器官代谢物的差异,对都匀毛尖本地茶树的叶、茎和根的代谢产物进行定性定量分析,鉴定出504个代谢物。采用主成分分析、正交偏最小二乘判别分析和差异倍数,筛选出叶与根205个差异代谢物,在根中下调的差异代谢物主要参与黄酮和黄酮醇生物合成、类黄酮生物合成、花青素生物合成等,主要决定茶汤的涩味,咖啡碱使茶叶具有辛苦味,氨基酸使茶叶具有鲜爽味,各种糖类代谢,增加茶的甜味。叶与茎的112个差异代谢物主要富集在谷胱甘肽、精氨酸和脯氨酸代谢、戊糖和葡萄糖醛酸转换等通路中。In order to study the metabolites difference in different organs of native tea plant Duyun Maojian,through qualitative and quantitative analysis on the metabolites among leaf,stem and root,a total of 504 metabolites were identified,and 205 different metabolites between leaf and root were obtained by principal component analysis(PCA),orthogonal partial least squares discrimination analysis(OPLS-DA),and fold change(FC).The down-regulated metabolites in root were mainly involved in flavonoid biosynthesis,flavonol biosynthesis,and anthocyanin biosynthesis et al,which mainly determine the astringency of the tea.Caffeine,amino acids and various sugar metabolisms made tea taste bitter,fresh,and sweet,respectively.The 112 differential metabolites between leaf and stem were mainly enriched in glutathione,arginine and proline metabolism,pentose and glucuronic acid conversion pathways.

关 键 词：都匀毛尖本地种茶树 代谢组 茶叶品质 

分 类 号：S571.1[农业科学—茶叶生产加工]