Urantide对动脉粥样硬化大鼠脾组织中ERK1/2及P38表达的影响  

Effect of Urantide on the expression of ERK1/2 and P38 in the spleen of atherosclerotic rats

作　　者：胥玉行 李英 谢云鹏[2] 王途 崔海鹏[1] 赵娟[1] Xu Yuhang;Li Ying;Xie Yunpeng;Wang Tu;Cui Haipeng;Zhao Juan(Department of Pathophysiology,Chengde Medical University,Chengde 067000,China;Affiliated Hospital of Chengde Medical University,Chengde 067000,China)

机构地区：[1]承德医学院病理生理学教研室,河北承德067000 [2]承德医学院附属医院,河北承德067000

出　　处：《中国临床解剖学杂志》2022年第4期425-431,共7页Chinese Journal of Clinical Anatomy

基　　金：河北省自然科学基金(H2020406011);河北省委组织部青年拔尖人才项目(冀组字[2016]9号);河北省科技厅科技创新引导专项;河北省教育厅重点项目(ZD2019098);承德医学院国家自然科学基金项目培育基金(201916);承德医学院自然科学青年基金培育基金(KY202123);河北省教育厅高校重点学科建设项目(冀教高[2013]4号病理学与病理生理学);承德医学院2022年基本科研业务费课题(KY202227)。

摘　　要：目的观察Urantide对动脉粥样硬化(atherosclerosis,AS)大鼠脾组织中MAPK信号通路ERK1/2和P38蛋白表达的影响,探究Urantide抗AS大鼠脾损伤的作用及机制。方法180只Wistar大鼠随机分为:正常组,模型组,辛伐他汀组,Urantide 3 d、7 d、14 d组。HE染色观察大鼠胸主动脉、脾组织;免疫组织荧光染色、Western blot检测大鼠脾中ERK1/2、P38蛋白表达。结果AS组与正常组相比,大鼠胸主动脉内出现典型AS病变,脾中央动脉出现玻璃样变,大鼠脾中p-ERK1/2、p-P38蛋白阳性表达升高(P<0.05)。Urantide各组与AS组相比,脾组织中玻璃样病变减轻,脾内p-ERK1/2、p-P38蛋白阳性表达降低,其中以Urantide 14 d组最明显(P<0.05)。结论Urantide可通过抑制ERK1/2、P38的表达,发挥抗AS作用,进而改善AS大鼠脾组织病变程度。Objective To observe the effect of Urantide on the expression of ERK1/2 and P38 in MAPK signaling pathway in spleen of atherosclerotic rats,and to explore the effect and mechanism of Urantide on ERK1/2 and P38 MAPK signaling pathway in spleen.Methods A total of 180 wistar rats were randomly divided into a normal group,an AS group,a Simvastatin group,a Urantide 3 days,7 days and 14 days group.The thoracic aorta and spleen of rats were observed by HE staining.Immunofluorescence,Western blotting were used to detect the expression of ERK1/2 and P38 in the spleen of rats.Results Compared with control group,the HE staining of AS group showed typical AS pathological changes in thoracic aorta,and hyaline degeneration in the central artery of spleen,the positive expression of p-ERK1/2 and p-P38 protein in the spleen of rats significantly increased(P<0.05).Compared with AS group,the hyaline degeneration in spleen significantly reduced in Urantide groups and Simvastatin group,the positive expression of p-ERK1/2 and p-P38 protein in spleen significantly decreased,especially in the Urantide 14 days group(P<0.05).Conclusions Urantide can improve the degree of pathological changes in spleen of AS rats by inhibiting the expression of ERK1/2 and P38.

关 键 词：动脉粥样硬化 脾 尾加压素Ⅱ URANTIDE MAPK ERK1/2 P38 

分 类 号：R543.5[医药卫生—心血管疾病]