正交实验法优选黑曲霉发酵柴胡黄芩药对的发酵工艺  

The Optimum Fermentation Conditions of Radix Scutellariae Radix by Aspergillus Niger Selected by Orthogonal Test

作　　者：田健[1] 孟凡娟 白健琳 郭远达 曲雁斌 金宇昕 TIAN Jian;MENG Fan-juan;BAI Jian-lin;GUO Yuan-da;QU Yan-bin;JIN Yu-xin(Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Women and Children Health Hospital,Changchun 130061,China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林省妇幼保健院,吉林长春130061

出　　处：《长春师范大学学报》2022年第6期70-73,99,共5页Journal of Changchun Normal University

基　　金：2021年度吉林省中医药科技项目“基于黑曲霉发酵柴胡黄芩复方含量变化的研究”(2021133)。

摘　　要：通过实验优选黑曲霉发酵柴胡黄芩药对的发酵工艺。以药对的有效成分总黄酮为指标,采用正交实验设计,对液料比、温度及初始含水量进行考察,确定最佳发酵工艺。最佳工艺为:柴胡黄芩药对中加入40%药材质量的水分,液料比为1∶1,在28℃下恒温培养。此方法对柴胡黄芩药对的有效成分得率高,工艺操作方便,外界对其影响相对较小。The fermentation process of aspergillus Niger and Scutellaria bupleurum was optimized by experiment.Taking the effective component of drug pair as comprehensive index,the ratio of liquid to material,temperature and water content were investigated through orthogonal experiment design to determine the best fermentation process.The best process is to add 40% of the weight of the medicinal material into the bupleurum baicalensis Georgi medicinepair.The liquid-solid ratio was 1∶1,and the culture was at a constant temperature of 28℃ under dark and constant temperature.This method has the advantages of high yield and convenient operation,and relatively little influence from outside.

关 键 词：正交实验法 黑曲霉 柴胡 黄芩 最优发酵条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]