外周血NK细胞、CD8^(+)T淋巴细胞水平预测早期卵巢癌患者腹腔镜术后淋巴结转移分析  

An analysis on NK cells and CD8^(+)T lymphocytes levels in peripheral blood for the prediction of lymph node metastasis in patients with early ovarian cancer after laparoscopic surgery

作　　者：曾海荣 黄丹 张建军 华海琴 ZENG Hairong;HUANG Dan;ZHANG Jianjun;HUA Haiqin(Department of Health Examination,Danzhou People′s Hospital,Danzhou,Hainan 571700,China;Department of Laboratory Medicine,Danzhou People′s Hospital,Danzhou,Hainan 571700,China;Department of Oncology,Danzhou People′s Hospital,Danzhou,Hainan 571700,China)

机构地区：[1]儋州市人民医院健康体检部,海南儋州571700 [2]儋州市人民医院检验科,海南儋州571700 [3]儋州市人民医院肿瘤科,海南儋州571700

出　　处：《国际检验医学杂志》2022年第14期1684-1688,共5页International Journal of Laboratory Medicine

基　　金：海南省卫生健康行业科研项目(18A200027);国家自然科学基金项目(81660433)。

摘　　要：目的观察外周血自然杀伤细胞(NK)、CD8^(+)T淋巴细胞在早期卵巢癌(OC)患者中的水平,并分析二者预测早期OC患者腹腔镜术后淋巴结转移的价值。方法回顾性分析2016年3月至2018年3月该院收治完成腹腔镜手术后3年内发生淋巴结转移的40例早期OC患者病历资料,设为转移组;另收集同期该院收治完成手术后3年内未发生淋巴结转移的40例早期OC患者资料,设为未转移组;均获得3年随访结果,随访时间截至2021年3月,查阅资料,记录患者基线资料及实验室指标,重点比较入院时外周血NK细胞、CD8^(+)T淋巴细胞水平,并分析二者预测早期OC患者术后淋巴结转移的价值。结果转移组患者入院时血清糖类抗原125(CA125)、人附睾蛋白-4(HE4)、外周血CD8^(+)T淋巴细胞水平高于未转移组,外周血NK细胞水平低于未转移组(P<0.05);回归分析发现,入院时HE4、CD8^(+)T淋巴细胞高水平、NK细胞低水平均可能与患者术后淋巴结转移有关(P<0.05);绘制受试者工作特征曲线,入院时NK细胞、CD8^(+)T淋巴细胞单独及联合预测早期OC患者术后淋巴结转移风险的曲线下面积均>0.80,有一定预测价值;相关性检验,外周血NK细胞、CD8^(+)T淋巴细胞水平之间呈负相关(r=-0.446,P<0.001)。结论外周血NK细胞低水平、CD8^(+)T淋巴细胞高水平对预测早期OC患者腹腔镜术后淋巴结转移风险具有一定价值。Objective To observe the levels of natural killer cells(NK)and CD8^(+)T lymphocytes in peripheral blood of patients with early ovarian cancer(OC),and to analyze the value of the two in predicting lymph node metastasis in patients with early OC after laparoscopic surgery.Methods A retrospective analysis was conducted,the medical data of 40 patients with early OC who had lymph node metastasis within 3 years after laparoscopic surgery in a hospital from March 2016 to March 2018 were collected and set as metastasis group;in addition,the data of 40 patients with early OC who did not have lymph node metastasis within 3 years after surgery in a hospital during the same period were collected and set as non-metastasis group.All patients were followed up for 3 years,the follow-up time was up to March 2021,the information was consulted,the baseline data and laboratory indexes of patients were recorded,the levels of NK cells and CD8^(+)T lymphocytes in peripheral blood at admission were mainly compared,the value of the two methods in predicting postoperative lymph node metastasis in patients with early OC was analyzed.Results The levels of serum carbohydrate antigen 125(CA125),human epididymal protein-4(HE4),CD8^(+)T lymphocytes in peripheral blood in the metastasis group were higher than those in the non-metastasis group,and the levels of NK cells in peripheral blood were lower than those in the non-metastasis group(P<0.05).Regression analysis showed that the high level of HE4,CD8^(+)T lymphocytes and low level of NK cells at admission might be related to postoperative lymph node metastasis of patients(P<0.05);The ROC curve was drawn,the AUC of NK cells,CD8^(+)T lymphocytes at admission alone or combined to predict the risk of postoperative lymph node metastasis in patients with early OC were all greater than 0.80,which had certain predictive value;through correlation test,there was a negative correlation between NK cells and CD8^(+)T lymphocytes in peripheral blood(r=-0.446,P<0.05).Conclusion Low level of NK cells and high

关 键 词：早期卵巢癌 自然杀伤细胞 CD8^(+)T淋巴细胞 腹腔镜手术 淋巴结转移 

分 类 号：R735.35[医药卫生—肿瘤]