陆地棉主要育种性状SSR关联位点的验证及优异材料鉴定  

Verification of SSR-associated loci for main breeding traits and identification of excellent germplasms in upland cotton(Gossypium hirsutum L.)

作　　者：张素君[1] 李兴河 王海涛 唐丽媛[1] 蔡肖[1] 刘存敬[1] 张香云[1] 张建宏[1] Zhang Sujun;Li Xinghe;Wang Haitao;Tang Liyuan;Cai Xiao;Liu Cunjing;Zhang Xiangyun;Zhang Jianhong(Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Cotton Biology and Genetics and Breeding in Huanghuai-Hai Semi-arid Region,National Cotton Improvement Center Hebei Branch,Shijiazhuang 050051,China)

机构地区：[1]河北省农林科学院棉花研究所/农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室/国家棉花改良中心河北分中心,石家庄050051

出　　处：《棉花学报》2022年第2期120-136,共17页Cotton Science

基　　金：河北省农林科学院科技创新专项(2022KJCXZX-MHS-1);河北省自然科学基金青年基金(C2021301039);棉花生物学国家重点实验室开放课题(CB2021A31);河北省农林科学院基本科研业务费基金项目(2021070204)

摘　　要：【目的】评价与陆地棉纤维品质、产量、黄萎病抗性等主要育种性状关联的简单重复序列(simple sequence repeat,SSR)位点的选择效果,并挖掘优异典型材料。【方法】在474份陆地棉中,利用双尾t-检验在26个关联标记中筛选具有显著筛选效果的变异位点,并鉴定典型材料。【结果】共检测到30个多态性位点,基于上述位点可将其中420份陆地棉材料完全区分。20个变异位点与目标性状显著(P<0.05)关联,其中,与铃重和纤维长度密切关联的变异位点各6个,与马克隆值、伸长率、株高和铃数关联的位点分别有3、3、1和1个。获得了与铃重(JESPR220-160+BNL2440-240+BNL226-320)和纤维长度(CM45-140+CGR6528-180+JESPR152-125)密切关联的分子标记组合各1个,表型效应分别达0.27 g和1.27 mm,根据上述标记位点鉴定优异典型材料14份。【结论】获得了具有显著筛选效果的多态性位点及优异典型材料,可为棉花分子标记辅助选择及组合选配提供参考。[Objective]This study aims to evaluate and validate SSR(simple sequence repeat)markers associated with traits of fiber quality,yield,and resistance to Verticillium wilt of upland cotton,and identify typical materials carrying elite alleles.[Method]Among the 26 SSR markers identified from previous studies,the two-tailed t-test was used to screen the mutation sites with significant effect on phenotypic variations of 474 upland cotton materials,and to identify typical materials.[Result]A total of 30 polymorphic loci were detected,and can be used to fully differentiate 420 upland cotton materials.Interestingly,20mutation sites were significantly associated with target traits(P<0.05),of which,6 mutation sites were closely related to boll weight and fiber length,respectively.Moreover,there are 3,3,1,and 1 loci associated with micronaire value,breaking elongation,plant height and boll number,respectively.And,the marker combinations(JESPR220-160+BNL2440-240+BNL226-320)and(CM45-140+CGR6528-180+JESPR152-125)were associated with the boll weight and fiber length,with phenotypic effect of 0.27 g and 1.27 mm,respectively.Furthermore,14 typical materials carrying elite alleles were obtained.[Conclusions]In this study,polymorphic sites with significant phenotypic effects and typical materials of elite alleles were obtained,which provided references for cotton molecular marker-assisted selection and material selection.

关 键 词：陆地棉 表型性状 SSR位点 筛选效果 典型材料 

分 类 号：S562[农业科学—作物学]