2型糖尿病大鼠非酒精性脂肪肝模型的建立  

Establishment of non-alcoholic fatty liver model in type 2 diabetic rats

作　　者：赵燕[1] 谭洪[1] 李会芳[1] 吕琼花 杜思成[2] 杨秋萍[2] ZHAO Yan;TAN Hong;LI Huifang;LYU Qionghua;DU Sicheng;YANG Qiuping(Department of Diabetes,the First Affiliated Hospital of Kunming Medical University,Yunnan Province,Kunming 650032,China;Department of Geriatrics,the First Affiliated Hospital of Kunming Medical University,Yunnan Province,Kunming 650032,China)

机构地区：[1]昆明医科大学第一附属医院糖尿病科,云南昆明650032 [2]昆明医科大学第一附属医院老年医学科,云南昆明650032

出　　处：《中国当代医药》2022年第20期48-51,F0003,共5页China Modern Medicine

基　　金：云南省科技计划项目(2013FZ270)。

摘　　要：目的建立2型糖尿病大鼠非酒精性脂肪肝模型,探讨2型糖尿病对大鼠肝脏的影响。方法健康雄性SD大鼠40只,随机分为正常对照(NC)组和糖尿病(DM)组,每组各20只。DM组采用腹腔内小剂量链脉菌素(STZ,35 mg/kg)注射联合高糖高脂饮食诱导建立2型糖尿病大鼠模型。成模后,检测大鼠血糖、三酰甘油(TG)、总胆固醇(TC)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肝指数、胰岛素敏感指数(ISI)、胰岛素抵抗指数(HOMA-IR)等胰岛素抵抗相关指标,大鼠肝组织HE染色并观察其病理学改变。结果造模成功6、7、8、9、10周后,DM组的体重低于NC组,血糖高于NC组,差异有统计学意义(P<0.05)。DM组的TG、TC、ALT、肝指数高于NC组,差异有统计学意义(P<0.05)。两组的AST比较,差异无统计学意义(P>0.05)。造模成功6、10周后,DM组的空腹胰岛素(FINS)、HOMA-IR高于NC组,ISI低于NC组,差异有统计学意义(P<0.05)。在光镜下观察到NC组正常肝细胞有序,肝小叶规律正常,细胞质大圆,细胞质均匀,没有脂肪变性和炎症细胞渗透。DM组肝细胞组织不当,脂肪变性和肝细胞水肿,炎症细胞渗透,部分斑点和局部坏死。DM组的非酒精性脂肪肝病活动度积分(NAS)高于NC组,差异有统计学意义(P<0.05)。结论低剂量腹腔注射对2型糖尿病大鼠结合高糖高脂肪可诱发非酒精性脂肪肝模型。Objective To establish a non-alcoholic fatty liver model in rats with type 2 diabetes,and to explore the effect of type 2 diabetes on the liver of rats.Methods Forty healthy male SD rats were randomly divided into normal control group(NC)and diabetic group(DM),with 20 rats in each group.Type 2 diabetic rats in DM group were induced by intraperitoneal injection of streptozotocin(STZ,35 mg/kg)combined with high-glucose and high-fat diet.After modeling,blood glucose,triacylglyceride(TG),total cholesterol(TC),alanine aminotransferase(ALT),aspartate aminotransferase(AST),liver index,insulin sensitive index(ISI),Homeostasismodel assessment insulin resistance index(HOMA-IR)related indicators were detected.Liver tissue of rats was stained with HE and its pathological changes were observed.Results At 6,7,8,9,10 weeks after modeling,the body weight of DM group was lower than that of NC group,and blood glucose was higher than that of NC group,the differences were statistically significant(P<0.05).TG,TC,ALT and liver index in DM group were higher than those in NC group,the differences were statistically significant(P<0.05).There was no significant difference in AST between the two groups(P>0.05).6,10 weeks after modeling,the fasting insulin(FINS),HOMA-IR of DM group were higher than those of NC group,ISI was lower than that of NC group,the differences were statistically significant(P<0.05).Under the light microscope,the normal liver cells in NC group were orderly,the liver lobules were normal,the cytoplasm was round and uniform,and there was no infiltration of steatosis and inflammatory cells.In DM group,liver cells were improperly organized,fatty degeneration,liver cell edema,infiltration of inflammatory cells,partial spots and local necrosis.The activity score of non-alcoholic fatty liver disease(NAS)in DM group was higher than that in NC group,and the difference was statistically significant(P<0.05).Conclusion Intraperitoneal injection of low-dose STZ combined with high-sugar and high-fat feeding can induce a non-alcoholi

关 键 词：大鼠 2型糖尿病 非酒精性脂肪肝 胰岛素抵抗指数 肝指数 

分 类 号：R575.5[医药卫生—消化系统]