克氏原螯虾CSGalNAcT-1基因功能初步研究  

Preliminary study on function of CSGalNAcT-1 gene in red swamp crayfish,Procambarus clarkii

作　　者：欧阳号锋 易少奎 张龙 杨思琦 李艳和 OUYANG Haofeng;YI Shaokui;ZHANG Long;YANG Siqi;LI Yanhe(College of Fisheries/Key Lab of Freshwater Animal Breeding,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China;College of Life Sciences,Huzhou University,Huzhou 313000,China;Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院/农业农村部淡水动物育种重点实验室,武汉430070 [2]湖州师范学院生命科学学院,湖州313000 [3]教育部长江经济带大宗水生生物产业绿色发展工程研究中心,武汉430070

出　　处：《华中农业大学学报》2022年第4期233-239,共7页Journal of Huazhong Agricultural University

基　　金：湖北省重点研发计划项目(2021BBA232);中央高校基本科研业务费专项(2662020SCPY004);国家自然科学基金青年基金项目(31501858)。

摘　　要：为探究硫酸软骨素N-乙酰半乳糖胺基转移酶-1(chondroitin sulfate N-acetylgalactosaminyltransferase-1,CSGalNAcT-1)在克氏原螯虾免疫反应中的作用,利用RACE技术成功获得克氏原螯虾CSGalNAcT-1基因完整cDNA序列,并通过荧光定量PCR分析该基因在各组织中的表达模式以及在CpG寡脱氧核苷酸(ODN)、嗜水气单胞菌和白斑综合征病毒(WSSV)刺激后的表达情况。结果显示,克氏原螯虾CSGalNAcT-1基因在中肠的相对表达量最高,其次是近胃段肠,在心脏、胃、肌肉和围食道神经中的相对表达量较低。克氏原螯虾肝胰腺、血细胞中CSGalNAcT-1基因表达量在嗜水气单胞菌和WSSV刺激下呈现出下调及上调,而在注射免疫刺激剂CpG ODN后,CSGalNAcT-1基因表达水平在大部分组织中呈现上调。表明克氏原螯虾CSGalNAcT-1基因可能在抵御外界病原侵染以及增强自身免疫反应的过程中发挥重要作用。To investigate the role of chondroitin sulfate N-acetylgalactosaminyltransferase-1(CSGalNAcT-1)played in the immune response of Procambarus clarkii,in this study,the complete cDNA sequence of the P.clarkii CSGalNAcT-1 was obtained by RACE,and its expression patterns in response to stimulation of CpG oligodeoxynucleotide(ODN),Aeromonas hydrophila and white spot syndrome virus(WSSV)were investigated by real-time PCR.The results indicated that the relative expression of CSGalNAcT-1 was highest in the midgut,followed by the foregut,and less in the heart,stomach,muscle and periesophageal nerve in P.clarkii.Besides,the expression of CSGalNAcT-1 in the hepatopancreas and haemocytes which were considered as immune organs of crustacean,could be down-regulated and up-regulated by challenges of A.hydrophila and WSSV,respectively,and the expression was up-regulated in most of the tissues after injected with CpG ODN,indicating that the CSGalNAcT-1 gene may play an important role in resisting the pathogenic infection and enhancing the autoimmune response in P.clarkii.

关 键 词：克氏原螯虾 CSGalNAcT-1基因 相对表达 白斑综合征病毒(WSSV) CpG ODN 嗜水气单胞菌 

分 类 号：Q78[生物学—分子生物学] S917.4[农业科学—水产科学]