岩溶与非岩溶区水稻土团聚体细菌群落结构和功能类群比较  

Comparison of Bacterial Community Structure and Functional Groups of Paddy Soil Aggregates Between Karst and Non-karst Areas

作　　者：肖筱怡 靳振江[1,2,3] 冷蒙 李雪松 熊丽媛 XIAO Xiao-yi;JIN Zhen-jiang;LENG Meng;LI Xue-song;XIONG Li-yuan(College of Environmental Science and Engineering,Guilin University of Technology,Guilin 541004,China;Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541004,China;Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]桂林理工大学环境科学与工程学院,桂林541004 [2]桂林理工大学岩溶地区水污染控制与用水安全保障协同创新中心,桂林541004 [3]桂林理工大学广西环境污染控制理论与技术重点实验室,桂林541004

出　　处：《环境科学》2022年第7期3865-3875,共11页Environmental Science

基　　金：国家自然科学基金项目(41867008);广西自然科学基金项目(2018GXNSFAA281247);广西科技计划项目(桂科AB21196050,桂科AD18126018)。

摘　　要：研究岩溶与非岩溶区土壤团聚体中微生物群落结构和功能类群异同,可以为揭示岩溶区土壤有机微生物与团粒结构稳定之间的互作机制提供理论依据.以岩溶与非岩溶稻田的耕层土壤为研究对象,采用湿筛法将团聚体分为大团聚体(0.25~2 mm)、微团聚体(0.053~0.25 mm)和粉、黏粒组分(<0.053 mm),通过高通量测序对土壤团聚体微生物群落结构进行分析,并采用共现网络分析细菌关键类群以及利用FAPROTAX进行功能预测.结果表明,(1)岩溶区大团聚体质量占比为30.94%,团聚体平均质量直径和几何平均直径分别为0.41 mm和0.15 mm,这3个项目均显著高于非岩溶区,表明岩溶区的环境有利于团聚体的形成;(2)岩溶区3个粒径团聚体的酸杆菌门、变形菌门、酸杆菌(Gp3、Gp4和Gp6)和鞘氨醇单胞菌属等相对丰度均高于非岩溶区.岩溶区微团聚体中的绿弯菌门的相对丰度(6.13%)显著高于粉、黏粒组分(2.79%);(3)共现网络分析表明,变形菌门和酸杆菌门为3个粒径团聚体中的关键类群.岩溶区微团聚体中细菌正相关边(65.5%)最高,相互关系多为协同关系;(4)岩溶区土壤3个粒径团聚体中化能异养类菌(15.52%、15.30%和16.89%)和需氧化能异养类菌(14.62%、14.38%和15.97%)为最优势功能类群,且相对丰度均显著高于非岩溶区.岩溶区大团聚体几丁质分解类菌相对丰度(0.46%)显著高于粉、黏粒组分(0.39%).结果表明,岩溶区的土壤环境能提高团聚体稳定性,且岩溶区的3个粒径团聚体中的细菌群落结构、关键类群和功能类群也与非岩溶区的有显著差异.Studying the similarities and differences in microbial community structure and functional groups in soil aggregates between karst areas(KA)and non-karst areas(NKA)can provide a theoretical basis for revealing the interaction mechanism between soil organic microorganisms and soil aggregate structure stability in KA.The surface soils(0-20 mm)were collected from paddy fields both in KA and NKA.The aggregates were divided into macro-aggregates(Mac,0.25-2 mm),micro-aggregates(Mic,0.053-0.25 mm),and silt-clay fractions(SC,<0.053 mm)using the wet sieving method.The microbial community structure of soil aggregates was analyzed using high-throughput sequencing.The keystone taxa were analyzed by the co-occurrence network,and their functions were predicted by FAPROTAX.The results showed that:(1)the proportion of Mac,mean weight diameter,and geometric mean diameter of aggregates in KA were 30.94%,0.41 mm,and 0.15 mm,respectively.These three factors in KA were significantly higher than those in NKA,indicating that the environment in the KA was conducive to the formation of aggregates.(2)The relative abundances of Acidobacteria,Proteobacteria,Acidobacteria(Gp3,Gp4,and Gp6),and Sphingomonas in three particle size aggregates in KA were higher than those in NKA.The relative abundance of Chloroflexi(6.13%)in Mic in KA was significantly higher than that in SC(2.79%).(3)Co-occurrence network analysis showed that Proteobacteria and Acidobacteria were keystone taxa in three particle size aggregates.The positive correlation edge(65.5%)of bacteria in KA was the highest,and most of the correlation was synergistic.(4)Chemoheterotrophy(15.52%,15.30%,and 16.89%)and aerobic chemoheterotrophy(14.62%,14.38%,and 15.97%)were the most dominant functional groups in the three particle size aggregates in KA,and their relative abundance was significantly higher than those in NKA.The relative abundance of chitinolysis in Mac in KA(0.46%)was significantly higher than that in SC(0.39%).The results showed that the soil environment in KA can improve the s

关 键 词：岩溶区 稻田 土壤团聚体 细菌群落 关键类群 功能类群 

分 类 号：X172[环境科学与工程—环境科学]