艾草黄酮提取工艺优化及抗氧化活性研究  

Optimization of extraction process and antioxidant activity of flavonoids from Artemisia argyi

作　　者：王娜[1,2,3] 张昊宇 宁灿灿 范允涛 王林青 余秋颖 WANG Na;ZHANG Hao-yu;NING Can-can;FAN Yun-tao;WANG Lin-qing;YU Qiu-ying(College of Food Science and Technology,Henan Agricultural University,Zhengzhou,Henan 450002,China;Zhengzhou Nutrition and Health Food Laboratory,Zhengzhou,Henan 450002,China;Key Laboratory of Bulk Grain Processing,Ministry of Agriculture and Rural Affairs,Zhengzhou,Henan 450002,China;Fengsenyuan Forestry Technology Co.,Ltd.,Beijing 102100,China;Key Laboratory of Molecular Biology,Zhengzhou Normal University,Zhengzhou,Henan 450044,China)

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450002 [2]郑州市营养与健康食品重点实验室,河南郑州450002 [3]农业农村部大宗粮食加工重点实验室,河南郑州450002 [4]北京丰森源林业科技有限公司,北京102100 [5]郑州师范学院分子生物学重点实验室,河南郑州450044

出　　处：《食品与机械》2022年第7期174-179,240,共7页Food and Machinery

基　　金：“十三五”国家重点研发专项(编号:2019YFC1605700);河南省科技攻关项目(编号:212102110324)。

摘　　要：目的:优化艾草黄酮提取工艺,并评价艾草黄酮抗氧化活性。方法:以艾草总黄酮得率、DPPH自由基清除率、OH自由基清除率为指标,确定艾草黄酮的提取方法;在单因素试验和Plackett-Burman试验基础上,通过响应面试验优化了超声—微波辅助水提法提取艾草黄酮的工艺条件。结果:最佳提取工艺条件为60℃水浴40 min,340 W超声27 min,600 W微波120 s,料液比1∶30(g/mL);该条件下艾草总黄酮得率可达87.93 mg/g,DPPH自由基清除率为80.84%,OH自由基清除率为77.92%。结论:该提取方法艾草黄酮的得率显著优于传统煎煮和水浴加热提取法(P<0.05),且具有较好的抗氧化活性。Objective:To optimize the extraction process of flavonoids from Artemisia argyi and evaluate the antioxidant activity in vitro.Methods:The ultrasonic-microwave combined water extraction method of Artemisia flavonoids was determined,and the extraction process was studied with the yield of total flavonoid,DPPH radical scavenging rate and·OH radical scavenging rate of Artemisia argyi.Based on the single factor experiment,the factors with greater influence were screened through Plackett-Burman design,then optimized extraction process by response surface test.Results:The best extraction process was ultrasonic power 340 W,ultrasonic time 27 min,microwave power 600 W,microwave time 120 s,solid-liquid ratio 1∶30(g/mL).Under these conditions,the yield of total flavonoids can reach 87.93 mg/g,DPPH radical scavenging rate is 80.84%,·OH radical scavenging rate is 77.92%.Conclusion:The yield of flavonoids by this method is significantly better than the traditional boiling and water bath extraction(P<0.05),and the antioxidant activity of Artemisia argy is better.

关 键 词：超声波辅助水提法 艾草黄酮 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]