Recent advances in RNA structurome  

作　　者：Bingbing Xu Yanda Zhu Changchang Cao Hao Chen Qiongli Jin Guangnan Li Junfeng Ma Siwy Ling Yang Jieyu Zhao Jianghui Zhu Yiliang Ding Xianyang Fang Yongfeng Jin Chun Kit Kwok Aiming Ren Yue Wan Zhiye Wang Yuanchao Xue Huakun Zhang Qiangfeng Cliff Zhang Yu Zhou 

机构地区：[1]MOE Laboratory of Biosystems Homeostasis&Protection,Innovation Center for Cell Signaling Network,College of Life Sciences,Zhejiang University,Hangzhou 310058,China [2]Key Laboratory of RNA Biology,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [3]Life Sciences Institute,Zhejiang University,Hangzhou 310058,China [4]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310058,China [5]State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan 430072,China [6]Beijing Advanced Innovation Center for Structural Biology,School of Life Sciences,Tsinghua University,Beijing 100084,China [7]Stem Cell and Regenerative Biology,Genome Institute of Singapore,A*STAR,Singapore,Singapore [8]Department of Chemistry,and State Key Laboratory of Marine Pollution,City University of Hong Kong,Hong Kong,China [9]MOE Key Laboratory of Bioinformatics,Beijing Advanced Innovation Center for Structural Biology and Frontier Research Center for Biological Structure,Center for Synthetic and Systems Biology,School of Life Sciences,Tsinghua University,Beijing 100084,China [10]Tsinghua-Peking Center for Life Sciences,Beijing 100084,China [11]Department of Cell and Developmental Biology,John Innes Centre,Norwich Research Park,Norwich NR47UH,United Kingdom [12]Shenzhen Research Institute of City University of Hong Kong,Shenzhen 518057,China [13]University of Chinese Academy of Sciences,Beijing 100101,China [14]Key Laboratory of Molecular Epigenetics of the Ministry of Education,Northeast Normal University,Changchun 130024,China

出　　处：《Science China(Life Sciences)》2022年第7期1285-1324,共40页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2021YFE0114900);the National Natural Science Foundation of China(91940303,91940306,32025008,32170262,31922039,U1832215,32170229);the Natural Science Foundation of Zhejiang Province(LD21C050002);the Starry Night Science Fund at Shanghai Institute for Advanced Study of Zhejiang University(SN-ZJU-SIAS-009);the Beijing Advanced Innovation Center for Structural Biology,Shenzhen Basic Research Project(JCYJ20180507181642811);Research Grants Council of the Hong Kong SAR,China Projects(City U 11100421,City U 11101519,City U 11100218,N_City U110/17);Croucher Foundation Project(9509003);State Key Laboratory of Marine Pollution Director Discretionary Fund,City University of Hong Kong Projects(7005503,9667222,9680261);the United Kingdom Biotechnology and Biological Sciences Research Council(BBSRC:BBS/E/J/000PR9788)。

摘　　要：RNA structures are essential to support RNA functions and regulation in various biological processes. Recently, a range of novel technologies have been developed to decode genome-wide RNA structures and novel modes of functionality across a wide range of species. In this review, we summarize key strategies for probing the RNA structurome and discuss the pros and cons of representative technologies. In particular, these new technologies have been applied to dissect the structural landscape of the SARS-CoV-2 RNA genome. We also summarize the functionalities of RNA structures discovered in different regulatory layers-including RNA processing, transport, localization, and mRNA translation-across viruses, bacteria, animals, and plants. We review many versatile RNA structural elements in the context of different physiological and pathological processes(e.g., cell differentiation, stress response, and viral replication). Finally, we discuss future prospects for RNA structural studies to map the RNA structurome at higher resolution and at the single-molecule and single-cell level, and to decipher novel modes of RNA structures and functions for innovative applications.

关 键 词：RNA structurome high-throughput techniques GENOME-WIDE 3D structure RNA secondary structure SARS-CoV-2 DECODING FUNCTION 

分 类 号：Q522[生物学—生物化学]