Techniques for increasing the yield of stem cell-derived exosomes:what factors may be involved?  

作　　者：Zi-Yuan Feng Qing-Yi Zhang Jie Tan Hui-Qi Xie 

机构地区：[1]Laboratory of Stem Cell and Tissue Engineering,Orthopedic Research Institute,Med-X Center for Materials,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China

出　　处：《Science China(Life Sciences)》2022年第7期1325-1341,共17页中国科学（生命科学英文版）

基　　金：sponsored by the National Natural Science Foundation of China(31771065);Sichuan Science and Technology Program(2019JDRC0020);the“1.3.5 Program for Disciplines of Excellence,”West China Hospital,Sichuan University(ZYJC18002)。

摘　　要：Exosomes are nano-scale extracellular vesicles secreted by cells and constitute an important part in the cell-cell communication.The main contents of the exosomes include proteins,micro RNAs,and lipids.The mechanism and safety of stem cell-derived exosomes have rendered them a promising therapeutic strategy for regenerative medicine.Nevertheless,limited yield has restrained full explication of their functions and clinical applications.To address this,various attempts have been made to explore the up-and down-stream manipulations in a bid to increase the production of exosomes.This review has recapitulated factors which may influence the yield of stem cell-derived exosomes,including selection and culture of stem cells,isolation and preservation of the exosomes,and development of artificial exosomes.

关 键 词：EXOSOME stem cell YIELD therapy 

分 类 号：R318[医药卫生—生物医学工程]