低磷胁迫条件下大豆磷高效近等基因系相关酶活性的变化  

Changes of enzyme activities of near isogenic lines with high phosphorus-use efficiency under low phosphorus stress in soybean

作　　者：董蓉娇 彭进乔 尹元萍[2] 王天明 梁坤 杨晓菲 董文汉 张雅琼 梁泉[1] DONG Rong-jiao;PENG Jin-qiao;YIN Yuan-ping;WANG Tian-ming;LIANG Kun;YANG Xiao-fei;DONG Wen-han;ZHANG Ya-qiong;LIANG Quan(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Natural Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;Scientific and Technology Division,Yunnan Agricultural University,Kunming 650201,China;Yunnan University of Traditional Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南农业大学资环学院,昆明650201 [3]云南农业大学科技处,昆明650201 [4]云南中医药大学民族医药学院,昆明650500

出　　处：《西南农业学报》2022年第6期1289-1294,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学地区基金(32060723);国家自然科学地区基金(31360497)。

摘　　要：测定低磷胁迫条件下大豆近等基因系(NIL)根系和叶片中酸性磷酸酶(ACP)、过氧化物酶(POD)、核糖核酸酶(RNAse)、吲哚乙酸氧化酶(IAAO)活性的变化,为解析大豆根系高效吸收利用磷的机理提供依据。【方法】设置高磷(1 mmol/L)、低磷(0.5μmol/L)水培试验,培养15 d后取样,利用试剂盒分别测定2种供磷水平下磷高效相关根形态构型近等基因系(NIL)及其受体亲本BD2(CK)根系和叶片中的ACP、POD、RNAse、IAAO活性。【结果】低磷胁迫条件下,NIL根系ACP、POD、RNAse、IAAO活性均显著高于BD2(CK),分别提高19.91%、10.48%、21.71%、22.31%;NIL叶片ACP、POD、RNAse、IAAO活性均显著高于BD2(CK),分别提高10.47%、26.86%、15.94%、8.7%。NIL根部ACP、POD、RNase、IAAO活性低磷水平显著高于其高磷水平,分别提高31.66%、20.98%、21.93%、41.89%;NIL叶片ACP、POD、RNase、IAAO活性显著低磷水平高于其高磷水平,分别提高14.73%、23.4%、120.95%、18.52%。【结论】NIL在适应磷胁迫环境条件方面显著优于BD2(CK)。本研究大豆根际ACP、POD、RNase、IAAO活性可作为评价磷高效基因型大豆的重要指标。研究结果可为磷高效遗传研究或育种利用提供依据。【Objective】This study measured the changes of acid phosphatase(ACP),peroxidase(POD),ribonuclease(RNase)and indole acetic acid oxidase(IAAO)activities in roots and leaves of soybean near isogenic lines(NIL)under low phosphorus stress,so as to provide a basis for analyzing the mechanism of increased phosphorus absorption and utilization by soybean roots.【Method】High phosphorus(1 mmol/L)and low phosphorus(0.5μmol/L)hydroponic culture experiments were set up.The tested samples were taken after 15 days of culture.The activities of ACP,pod,RNase and IAAO in the roots and leaves of high phosphorus-use related root morphological configuration near isogenic line(NIL)and its receptor parent BD2(CK)under two phosphorus supply levels were measured by the corresponding kits.【Result】Under low phosphorus stress,the activities of ACP,POD,RNase and IAAO in roots of NIL were significantly higher than those in BD2(CK),increased by 19.91%,10.48%,21.71%and 22.31%respectively;The activities of ACP,POD,RNase and IAAO in the leaves of NIL were significantly higher than those in BD2(CK),increased by 10.47%,26.86%,15.94%and 8.7%respectively.The activities of ACP,POD,RNase and IAAO in roots of NIL under low P treatment were significantly higher than those of high P treatment,increased by 31.66%,20.98%,21.93%and 41.89%,respectively.The activities of ACP,POD,RNase and IAAO in leaves of NIL under low P treatment were significantly higher than those of high P treatment,increased by 14.73%,23.4%,120.95%and 18.52%,respectively.【Conclusion】NIL was significantly better than BD2(CK)in adapting to phosphorus deficiency.In this study,the activities of ACP,pod,RNase and IAAO in soybean rhizosphere can be used as important indexes to evaluate phosphorus use efficiency of genotypes of soybean.The results can provide a basis for high-efficiency genetic research or breeding utilization of phosphorus.

关 键 词：大豆 近等基因系 低磷胁迫 酶活性 

分 类 号：S565[农业科学—作物学]