基于UPLC-Triple-TOF-MS/MS结合SWATH技术对不同泌乳期人乳脂质变化分析  

Sequential Window Acquisition of All Theoretical Mass Spectra-Assisted Ultra-high Performance Liquid Chromatography-Triple-Time of Flight-Tandem Mass Spectrometry for Analysis of Changes in Human Milk Lipids during Different Lactation Periods

作　　者：王筱迪 周金杭 曹雪 高文浩 尹乾隆 任皓威[1,2] 刘宁 WANG Xiaodi;ZHOU Jinhang;CAO Xue;GAO Wenhao;YIN Qianlong;REN Haowei;LIU Ning(Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agricultural University,Harbin 150030,China;Harbin Tengning Technology Co.Ltd.,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]哈尔滨腾凝科技有限公司,黑龙江哈尔滨150030

出　　处：《食品科学》2022年第14期208-218,共11页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFC160430401)。

摘　　要：为深入了解婴儿时期人乳中复杂的脂质含量的动态变化,采用超高效液相色谱-三重四极杆串联飞行时间质谱及具有高采集命中率的顺序窗口采集所有理论质谱模式(sequential window acquisition of all theoretical mass spectra,SWATH)对不同泌乳阶段人乳中的脂质进行鉴定及相对定量分析。结果显示,在3个泌乳阶段的人乳中共检测到229种脂质。根据“脂质代谢途径研究计划”中的分类,共检测到五大类物质:甘油酯类(glycerolipid,GL)主要包括24种甘油二酯和28种甘油三酯;甘油磷脂类(glycerophospholipid,GP)主要包括33种磷脂酰乙醇胺、21种磷脂酰胆碱、8种磷脂酰丝氨酸、13种磷脂酰甘油、10种磷脂酸和7种磷脂酰肌醇;鞘脂类(sphingolipid,SP)化合物共22种;甾醇脂类(sterol lipid,ST)化合物共10种;脂肪酸类及其衍生物(fatty acids,FAs)共4种。随着泌乳期的延长,GL、SP的相对含量逐渐增加,而GP、ST、FAs的相对含量逐渐下降。本研究实现了人乳中脂质的全面定性与相对定量分析,方法灵敏度高、精密度及稳定性良好,同时搭配SWATH采集模式,为科学模拟各阶段人乳及开发不同段龄婴幼儿奶粉的研发提供一定参考依据。In order to understand the dynamic changes of complex lipid contents in human milk during infancy,sequential window acquisition of all theoretical mass spectra(SWATH)with high acquisition hit rate assisted ultra-high performance liquid chromatography-triple quadrupole time of flight tandem mass spectrometry(UPLC-Triple-TOF-MS/MS)was used to identify and quantitatively analyze lipids in human milk at different lactation stages.The results showed that a total of 229 lipids were detected in human milk at the three lactation stages.According to the“Lipid Metabolism and Pathways Strategy”,five classes of lipids were identified:1)glycerolipids(GLs)mainly including 24 diglycerides and 28 triglycerides;2)glycerophospholipids(GPs)including 33 phosphatidylethanolamines,21 phosphatidylcholines,8 phosphatidylserines,13 phosphatidylglycerols,10 phosphatidic acids and 7 phosphatidylinositols;3)22 sphingolipids(SP);4)10 sterol lipids(ST);and 5)4 fatty acids(FAs)and their derivatives.The relative contents of GL and SP increased gradually with lactation time,while the relative contents of GP,ST and FAs decreased gradually.This study achieved a comprehensive qualitative and relative quantitative analysis of lipids in human milk.The developed method is sensitive,precise and stable.It provides a basis for the development of infant food simulating human milk at different stages of lactation.

关 键 词：人乳 脂质 脂肪酸 顺序窗口采集所有理论质谱模式 脂质组学 

分 类 号：TS252.42[轻工技术与工程—农产品加工及贮藏工程]