胃癌组织神经元再生相关蛋白的表达及临床意义研究  

Expression of neuronal regeneration associated protein and its clinical significance in gastric cancer

作　　者：徐园 贾黎华 虞伟明[1] XU Yuan;JIA Lihua;YU Weiming(不详;Department of Gastrointestinal Surgery,Ningbo Medical Center Lihuili Hospital,Ningbo 315211,China)

机构地区：[1]宁波市医疗中心李惠利医院胃肠外科,315211 [2]金华市中医院外科

出　　处：《浙江医学》2022年第13期1368-1373,I0004,共7页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目(2022481676)。

摘　　要：目的探讨神经元再生相关蛋白(NREP)在胃癌组织中的表达及临床意义。方法选取2021年3月至8月在宁波市医疗中心李惠利医院接受D2根治术的20例胃癌患者的胃癌组织和对应的癌旁正常组织作为研究标本,采用在线生物信息数据库(Oncomine、GEPIA、Ualcan数据库)分析胃癌组织NREP mRNA的表达。采用基因芯片分析胃癌组织差异表达基因以验证NREP mRNA表达情况。采用人类蛋白质图谱(HPA)分析胃癌组织NREP蛋白的表达。免疫组化检测胃癌组织和癌旁正常组织NREP蛋白的表达。采用Kaplan-Meier plotter数据库分析胃癌患者NREP mRNA表达与预后的关系。结果Oncomine、GEPIA、Ualcan数据库分析显示,胃癌组织NREP mRNA表达水平高于癌旁正常组织(P<0.05)。基因芯片分析发现NREP mRNA在胃癌组织中高表达,其表达水平比癌旁正常组织高至少2.8倍(P<0.05)。胃癌组织NREP蛋白高表达比例高于癌旁正常组织(P<0.05)。多因素分析显示,NREP高表达是胃癌患者预后不良的独立危险因素之一(P<0.05)。NREP mRNA低表达胃癌患者总生存时间、无进展生存时间均优于高表达胃癌患者(均P<0.05)。结论NREP在胃癌组织中高表达,且其高表达与患者预后不良密切相关。NREP或可成为评估胃癌预后和指导治疗的生物标志物。Objective To investigate the expression of neuron regeneration related protein(NREP)in gastric cancer(GC)and its clinical significance.Methods The GC tissues and adjacent normal tissues were collected from 20 GC pa-tients who underwent D2 radical gastrectomy in Ningbo Medical Center Lihuili Hospital from March 2021 to August 2021.The expression of NREP protein in GC and adjacent normal tissues was detected by immunohistochemistry.The data of NREP mRNA expression in GC were obtained from online bioinformatics databases(Oncomine,GEPIA,and Ualcan).The differen-tially expressed genes in GC tissues were analyzed by gene microarray and Human Protein Atlas(HPA)were used to verify the expression of NREP.Kaplan Meier plotter database was used to analyze the relationship between NREP expression and prognosis in GC patients.Results Analysis based on Oncomine,GEPIA and Ualcan databases showed that the expres-sion level of NREP mRNA in GC tissues was higher than that in adjacent normal tissues(P<0.05).Gene microarray analysis showed that the expression level of NREP mRNA was 2.8-fold higher in GC tissues than that in adjacent normal tissues(P<0.05).The protein level of NREP in GC tissues was higher than that in adjacent normal tissues(P<0.05).Multivariate analysis showed that high expression of NREP was an independent risk factor for poor prognosis in patients with GC(P<0.05).The overall survival and progression-free survival of patients with low expression of NREP mRNA were better than those of pa-tients with high expression of NREP mRNA(all P<0.05).Conclusion NREP is highly expressed in GC,and its high expres-sion is closely associated with poor prognosis.NREP may be a potential biomarker for the prognosis and treatment of GC.

关 键 词：神经元再生相关蛋白 胃癌 生物信息学 预后 

分 类 号：R735.2[医药卫生—肿瘤]