香石竹DcERF-1转录因子对切花衰老的负调控作用  

The Negative Regulation of DcERF-1 on Senescence of Cut Carnation

作　　者：王妍 孙政 冯珊 袁心怡 仲林林 曾云流[1,2] 傅小鹏 程运江[1,2,3] 包满珠[1,4] 张帆 WANG Yan;SUN Zheng;FENG Shan;YUAN Xinyi;ZHONG Linlin;ZENG Yunliu;FU Xiaopeng;CHENG Yunjiang;Bao Manzhu;ZHANG Fan(Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;National R&D Center For Citrus Preservation,Wuhan 430070,China;Hubei Hongshan Laboratory,Wuhan 430070,China;Key Laboratory of Huazhong Urban Agriculture,Ministry of Agriculture and Rural Affairs,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070 [2]国家柑橘保鲜技术研发专业中心,武汉430070 [3]湖北洪山实验室,武汉430070 [4]华中农业大学园艺林学学院,农业农村部华中都市农业重点实验室,武汉430070

出　　处：《园艺学报》2022年第6期1313-1326,共14页Acta Horticulturae Sinica

基　　金：中央高校基本科研业务费项目(2662019PY049);华中农业大学高层次人才启动经费项目。

摘　　要：以香石竹(Dianthus caryophyllus L.)为材料,克隆得到1个ERF转录因子基因,命名为DcERF-1。氨基酸序列比对及系统进化树分析发现,DcERF-1与拟南芥AtERF-1的同源性最高,属于Ⅸ(B3)亚组。实时荧光定量PCR显示,DcERF-1表达量在花中最高,且在花瓣自然衰老和乙烯诱导的衰老过程中都呈现先上升后下降的趋势。DcERF-1定位于细胞核中。DcERF-1在香石竹花瓣中瞬时过量表达后,花瓣褪色速度明显延缓,离子渗透率明显降低;DcERF-1被瞬时沉默后,花瓣褪色速度显著加快,离子渗透率显著升高,衰老标志基因DcSAG12表达量显著上调。酵母单杂交试验证明乙烯信号转导途径核心转录因子DcEIN3能直接结合DcERF-1的启动子。双荧光素酶瞬时表达试验证明DcERF-1能抑制乙烯生物合成途径中ACC氧化酶基因DcACO4的表达活性。综合结果表明DcERF-1负调控香石竹切花衰老。In order to explore the mechanism of ethylene regulated flower senescence in cut carnation,we screened a transcription factor named DcERF-1 from the expression profile of carnation.Analysis of deduced amino acid sequence and phylogenetic tree found that DcERF-1 showed high homology with Arabidopsis thaliana AtERF-1,and belongs toⅨ(B3)sub-group.Real-time quantitative PCR showed that DcERF-1 was highly expressed in flower,and the expression trend of DcERF-1 was firstly increased and then decreased during both the natural flower senescence and ethylene induced flower senescence progress.The subcellular localization assay showed that DcERF-1 was localized in the nucleus.Transient overexpression of DcERF-1 in the petals resulted that the petal fading rate was significantly delayed and the ion leakage rate was significantly reduced.After transient silencing of DcERF-1,the petal fading rate was significantly accelerated,the ion leakage rate was significantly increased,and the expression of senescence marker gene DcSAG12 was significantly up-regulated.In addition,yeast one hybrid assay showed that DcEIN3 could directly bind the promoter of DcERF-1,and dual luciferase assay showed that DcERF-1 could inhibit the expression of DcACO4.The comprehensive results indicated that DcERF-1 negatively regulates the flower senescence in cut carnation.

关 键 词：香石竹 切花衰老 乙烯 采后 ERF转录因子 转录调控 

分 类 号：S681.5[农业科学—观赏园艺]