WT-PE:Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing  

作　　者：Rui Tao Yanhong Wang Yun Hu Yaoge Jiao Lifang Zhou Lurong Jiang Li Li Xingyu He Min Li Yamei Yu Qiang Chen Shaohua Yao 

机构地区：[1]From laboratory of Biotherapy,National Key Laboratory of Biotherapy,Cancer Center,West China Hospital,Sichuan university,Renmin Nanlu 17,Chengdu 610041 Sichuan,China

出　　处：《Signal Transduction and Targeted Therapy》2022年第5期1756-1763,共8页信号转导与靶向治疗（英文）

基　　金：We thank Yang Yang for providing python script for HTS data analysis.This work was supported by National Natural Science Foundation of China.No.U19A2002,No.81771220 and No.81974238.

摘　　要：Large scale genomic aberrations including duplication,deletion,translocation,and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of cancer.The current prime editor,PE2,consisting of Cas9-nickase and reverse transcriptase enables efficient editing of genomic deletion and insertion,however,at small scale.Here,we designed a novel prime editor by fusing reverse transcriptase(RT)to nuclease wild-type Cas9(WT-PE)to edit large genomic fragment.

关 键 词：Cas9 NUCLEASE EDITOR 

分 类 号：Q78[生物学—分子生物学]