2016—2020年辽宁省沙门菌耐药性及分子分型研究  

Drug resistance characteristics and molecular typing of Salmonella in Liaoning Province from 2016 to 2020

作　　者：耿英芝[1] 于淼[1] 张铭琰 张眉眉[1] GENG Yingzhi;YU Miao;ZHANG Mingyan;ZHANG Meimei(Liaoning Center for Disease Prevention and Control,Liaoning Shenyang 110005,China)

机构地区：[1]辽宁省疾病预防控制中心,辽宁沈阳110005

出　　处：《中国食品卫生杂志》2022年第3期426-431,共6页Chinese Journal of Food Hygiene

基　　金：辽宁省自然基金指导计划(2019-ZD-1089)。

摘　　要：目的分析辽宁省2016—2020年食源性疾病监测中沙门菌分离株的耐药情况及分子分型特征,为沙门菌引起感染性腹泻的防控、临床抗生素使用提供可靠科学依据。方法对辽宁省2016—2020年临床腹泻病例分离的90株沙门菌进行血清学鉴定,应用脉冲场凝胶电泳(PFGE)进行分子分型,采用BioNumeries 7.6软件对菌株间的相似度进行聚类分析,采用微量肉汤稀释法进行药物敏感性检测。结果90株沙门菌分为13种血清型,以肠炎沙门菌和鼠伤寒沙门菌为主。PFGE聚类分析得到54种带型,各带型间相似度为51.4%~100%,每种带型包含1~10株菌,同一血清型菌株的PFGE带型相似度较高,且存在多次聚集现象。药敏结果显示90株沙门菌呈现36种耐药谱,氨苄西林耐药率最高(66.7%,60/90),其次为萘啶酸(62.2%,56/90),头孢西丁全部敏感。多重耐药率达48.9%(44/90),其中鼠伤寒沙门菌和肠炎沙门菌多重耐药率分别为87.5%和45.8%。结论辽宁省沙门菌引起腹泻病例呈散发态势,菌株多重耐药趋势明显,且耐药率较高,耐药谱广泛,应进一步加强分子溯源及耐药性监测。Objective To investigate the molecular types and antimicrobial resistance of Salmonella isolated from foodborne disease surveillance in Liaoning Province during 2016—2020 and provide reliable scientific basis for the prevention and control of Salmonella infection and antibiotic treatment.Methods A total of 90 strains of Salmonella isolated from clinical diarrhea cases in foodborne disease surveillance in Liaoning Province from 2016 to 2020 were detected for drug sensitivity by broth dilution method.The molecular typing was analysed by pulsed-field gel electrophoresis(PFGE)method.Bionumeries 7.6 software was used to analyze the similarity between strains.Results The 90 Salmonella strains were divided into 13 serotypes,of which Salmonella typhimurium and Salmonella enteritidis were dominant.The 90 strains were divided into 54 PFGE patterns by Xba I.The similarity between each pattern was 51.4%~100%,including 1~10 strains.The same serotype strains had highly similar PFGE pattern,and there was multiple clustering phenomenon.The drug susceptibility results showed that 90 strains of Salmonella showed 36 kinds of drug resistance spectrum.The resistance rate to ampicillin was the highest(66.7%),followed by that to nalidixic acid(62.2%).All strains were sensitive to cefoxitin.The overall multi-drug resistance rate reached 48.9%(44/90),among which the predominant serotypes of Salmonella typhimurium and Salmonella enteritidis were 87.5%and 45.8%.Conclusion The cases of diarrhea caused by Salmonella in Liaoning Province are sporadic,but the trend of multi-drug resistance is obvious,the overall drug resistance rate is relatively high,and the drug resistance spectrum is wide.Traceability and drug resistance monitoring should be further strengthened.It is suggested that molecular tracing and drug resistance surveillance for Salmonella should be further strengthened.

关 键 词：辽宁省 沙门菌 血清分型 耐药性 分子分型 

分 类 号：R155[医药卫生—营养与食品卫生学]