莪术提取物通过mTOR/p70S6K信号通路调控胆囊癌细胞增殖、凋亡、侵袭和迁移的机制  

Mechanism of zedoary turmeric extract regulating the proliferation, apoptosis, invasion and migration of gallbladder cancer cells through the mTOR/p70S6K signaling pathway

作　　者：张大闯 董浩 马富平 吴胜利 刘宁 ZHANG Da-chuang;DONG Hao;MA Fu-ping;WU Sheng-li;LIU Ning(Second Department of Hepatobiliary,Xianyang Central Hospital,Xianyang 712000,China)

机构地区：[1]咸阳市中心医院肝胆二科,陕西咸阳712000

出　　处：《中国现代普通外科进展》2022年第7期505-509,共5页Chinese Journal of Current Advances in General Surgery

基　　金：陕西省重点研发计划项目(2020SF-060)。

摘　　要：目的:探讨莪术提取物(β-榄香烯)通过调控mTOR/p70S6K信号通路对胆囊癌细胞增殖、凋亡、侵袭和迁移的影响。方法:采取不同浓度(0、20、50、100μg/mL)的莪术提取物处理胆囊癌细胞GBC-SD,记为0μg/mL组、20μg/mL组、50μg/mL组、100μg/mL组;选取100μg/mL的莪术提取物和20μmoL/L的mTOR抑制剂CCI-779,记为莪术提取物组和莪术提取物+CCI-779组。采用MTT、流式细胞仪检测细胞的活力和凋亡;Transwell检测细胞的迁移和侵袭;Western blot检测Cleave-capase3、Bax、MMP2和mTOR/p70S6K信号通路相关蛋白的表达。结果:不同浓度的莪术提取物组可以降低细胞活力、细胞迁移数目和侵袭数目,增加细胞的凋亡率,上调Cleave-capase3、Bax蛋白表达,下调MMP2、p-mTOR、p-p70S6K蛋白表达,呈浓度依赖性,差异均有统计学意义(P<0.05)。莪术提取物+CCI-779组的细胞活力、细胞迁移数目、侵袭数目和MMP2蛋白表达低于莪术提取物组,细胞凋亡率、Cleave-capase3、Bax蛋白表达高于莪术提取物组,差异均有统计学意义(P<0.05)。结论:莪术提取物通过抑制mTOR/p70S6K信号通路抑制胆囊癌细胞增殖、侵袭和迁移,并促进其凋亡。Objective:To explore the effects of zedoary turmeric extract(β-elemene)the proliferation, apoptosis, invasion and migration of gallbladder cancer cells by regulating the mTOR/p70S6K signaling pathway. Methods: Different concentrations(0, 20, 50, 100 μg/mL) of zedoary turmeric extract were used to treat gallbladder cancer cells GBC-SD, which were recorded as 0 μg/mL group, 20 μg/mL group, 50 μg/mL group, 100 μg/mL group;the 100 μg/mL group of zedoary turmeric extract and 20 μmoL/L of mTOR signaling pathway inhibitor CCI-779 were selected, and they were recorded as zedoary turmeric extract group and zedoary turmeric extract+CCI-779 group. MTT and flow cytometry were used to detect cell viability and apoptosis;Transwell to detect cell migration and invasion;Weatern blot to detect the expression of Cleave-capase3, Bax, MMP2 and mTOR/p70S6K signaling pathway related proteins. Results: Different concentrations of zedoary turmeric extract group could reduce cell viability, cell migration number and invasion number, increase cell apoptosis rate, up-regulate the expression of Cleave-capase3 and Bax protein, and down-regulate the expression of MMP2, p-mTOR, and p-p70S6K protein, and it is concentration-dependent, the differences are statistically significant (P<0.05). The cell viability, cell migration number, invasion number and MMP2 protein expression of the zedoary turmeric extract+CCI-779 group were lower than those of the zedoary turmeric extract group, and the apoptosis rate, Cleave-capase3 and Bax protein expression were higher than those of the zedoary turmeric extract group the differences are statistically significant(P<0.05). Conclusion: Zedoary turmeric extract inhibits the proliferation, invasion and migration of gallbladder cancer cells by inhibiting the mTOR/p70S6K signaling pathway, and promotes their apoptosis.

关 键 词：莪术提取物 mTOR/p70S6K信号通路 胆囊癌细胞 增殖 凋亡 侵袭 迁移 

分 类 号：R735.8[医药卫生—肿瘤]