聚集素基因多态性与脑卒中后血管性痴呆的相关性分析  

Association analysis of aggregator gene polymorphism and vascular dementia after stroke

作　　者：马玥[1] 卢杉 张鹏 Ma Yue;Lu Shan;Zhang Peng(Department of Neurology,Heilongjiang Hospital,Haerbin Heilongjiang 150002;不详)

机构地区：[1]黑龙江省医院神经内科,哈尔滨150002 [2]黑龙江省医院医学影像科,哈尔滨150002 [3]黑龙江省医院老年神经内科,哈尔滨150002

出　　处：《卒中与神经疾病》2022年第3期215-219,共5页Stroke and Nervous Diseases

基　　金：黑龙江省卫生健康委科研课题(课题编号为2020-260)。

摘　　要：目的 探讨聚集素基因多态性与脑卒中后血管性痴呆的关系。方法 选取2019年12月-2020年12月本院收治的88例脑卒中后血管性痴呆患者作为观察组,并选取同期体检健康的60例健康者作为对照组,采用竞争性等位基因特异性聚合酶链反应(Kompetitive allele specific polymerase chain reaction,KASP)基因分型检测技术和基因测序法测定聚集素基因多态性(rs11136000和rs9331888位点基因亚型和等位基因分布频率),分析聚集素基因多态性与脑卒中后血管性痴呆的相关性。结果 2组基因分布符合哈代-温伯格(Hardy-Weinberg)平衡(F值分别为0.439和0.546,P>0.05),2组基因型分布频率达到基因遗传平衡标准,可体现人群基因分布情况,样本具有代表性,且观察组聚集素基因rs11136000位点TT基因型(1.14%)、TG基因型(32.95%)和T等位基因(43.18%)分布频率显著低于对照组的8.33%、50.00%、60.00%,而GG基因型(65.91%)和G等位基因(97.73%)分布频率显著显著高于对照组的40.00%和88.33%(P<0.05)。观察组聚集素基因rs9331888位点CC,CG,GG基因型和C,G等位基因分布频率与健康组比较无明显差异(P>0.05)。观察组合并糖尿病史和高脂血症史的比例和总胆固醇水平显著高于对照组,脑卒中后血管性痴呆与糖尿病史、总胆固醇水平、高脂血症史密切相关(P<0.05),与受教育年限、高血压病史、冠心病史、甘油三酯水平、婚姻状况、心律失常史、心肌梗死史、贫血史等因素无关(P>0.05)。对有统计学意义的相关因素进行多因素Logistic回归分析显示,糖尿病史(OR=1.677,95%CI=1.083~2.596)、总胆固醇水平(OR=1.587,95%CI=1.041~2.419)、携带GG基因型(OR=2.812,95%CI=1.341~5.900)、携带G等位基因(OR=2.863,95%CI=1.411~5.810)是脑卒中后血管性痴呆发生的独立危险因素(OR>1,P<0.05)。结论 聚集素基因rs11136000位点GG基因型、G等位基因分布频率与脑卒中后血管性痴呆发生相关,在脑卒中后血管性痴Objective To explore and analyze the association between aggregator gene polymorphism and vascular dementia after stroke.Methods 88 stroke patients were admitted from December 2019 to December 2020 as the observation group,and 60 healthy people during the physical examination as the control group.Competitive allele-specific PCR(Kompetitive Allele Specific PCR) was used as the control group.PCR,KASP genotyping detection technology and gene sequencing method to determine the polymorphism of agglutinin gene(rs11136000 and rs9331888 locus genotype and allele distribution frequency),analyze the polymorphism of agglutinin gene and post-stroke vascularity the relevance of dementia.Results The gene distribution of the two groups was in hardy-Weinberg equilibrium(F=0.439 and 0.546,P>0.05).The gene distribution frequency of the two groups reached the standard of genetic balance,which could reflect the population gene distribution and the sample was representative.The distribution frequencies of TT genotype(1.14%),TG genotype(32.95%) and T allele(43.18%) of rs11136000 locus of aggregator gene in observation group were significantly lower than those in control group(8.33%,50.00%,60.00%).The distribution frequency of GG genotype(65.91%) and G allele(97.73%) was significantly higher than control group(40.00%/88.33%),the difference was statistically significant(P0.05).Observe combination and diabetes cases and total cholesterol were significantly higher than control group,vascular dementia and diabetes history,after stroke is closely related to the total cholesterol levels and hyperlipidemia(P0.05).Multivariate Logistic regression analysis of statistically significant related factors showed that diabetes mellitus(OR=1.677,95%CI=1.083~2.596),total cholesterol(OR=1.587,95%CI=1.041~2.419),GG genotype(OR=2.812,95%CI=1.341~5.900),G allele(OR=2.863,95%CI=1.411~5.810) were independent risk factors for vascular dementia after stroke(OR>1,P<0.05).Conclusion The distribution frequency of GG genotype and G allele at rs11136000 locus of ag

关 键 词：聚集素基因 基因多态性 脑卒中 血管性痴呆 相关性 

分 类 号：R743.3[医药卫生—神经病学与精神病学]