黄芩素调控NLRP3/Caspase-1通路对牙周炎大鼠牙槽骨吸收的影响  

Baicalein Regulates NLRP3/Caspase-1 Pathway and Its Effect on Alveolar Bone Resorption in Rats with Periodontitis

作　　者：杜宁[1] 张哲 周湘[3] 李凯 刘昕[4] 刘学聪[1] DU Ning;ZHANG Zhe;ZHOU Xiang;LI Kai;LIU Xin;LIU Xuecong(Department of Stomatology,Hebei Children’s Hospital,Shijiazhuang,050031,China;Health Hebei Guidance Center,Hebei Provincial Health Commission,Shijiazhuang,050061,China;Office of Total Quality Control,Hebei Children’s Hospital,Shijiazhuang,050031,China;Department of Stomatology,the Fourth Hospital of Hebei Medical University,Shijiazhuang,050011,China)

机构地区：[1]河北省儿童医院口腔科,石家庄市050031 [2]河北省卫健委健康河北指导中心,石家庄市050061 [3]河北省儿童医院全面质量控制办公室,石家庄市050031 [4]河北医科大学第四医院口腔科,石家庄市050011

出　　处：《医学分子生物学杂志》2022年第4期276-281,共6页Journal of Medical Molecular Biology

基　　金：河北省卫生健康委员会(No.20211445)。

摘　　要：目的 探索黄芩素调控核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)/半胱氨酸天冬氨酸蛋白酶1(cysteine aspartate protease 1,Caspase-1)通路对牙周炎大鼠牙槽骨吸收的影响.方法 将40只牙周炎大鼠随机分为模型组、黄芩素组、激活剂组、黄芩素+激活剂组,另取10只正常作为对照组.检测大鼠釉牙骨质界到牙槽嵴顶(CEJ-AC)的距离、血清中白细胞介素-6(IL-6)、转化生长因子-β(TGF-β)含量以及牙周组织病理变化、IL-6、TGF-β阳性表达和NLRP3、Caspase-1蛋白表达.结果 模型组大鼠CEJ-AC、NLRP3、Caspase-1、IL-6、TGF-β水平及阳性表达水平以及蛋白表达水平均升高(P<0.05);经黄芩素干预后,各项指标均降低(P<0.05);引入激活剂明显削弱了黄芩素对牙周炎大鼠的抗炎作用.结论 黄芩素通过抑制NLRP3/Caspase-1通路减轻炎性反应,控制牙槽骨吸收.Objective To explore the effect of baicalein on alveolar bone resorption in rats with periodontitis and its regulation of nucleotide-binding oligomerization domain-like receptor protein 3/cysteine aspartate protease 1(NLRP3/Caspase-1)pathway.Methods A total of 40 rats with periodontitis were randomly divided into model group,baicalein group,activator group and baicalein+activator group,another 10 rats without any treatment were taken as the control group.The distance from the cemento-enamel junction to aleolar crest(CEJ-AC)of the rats were measured.The expressions of interleukin-6(IL-6)and transforming growth factor-β(TGF-β)in the serum and the periodontal tissues were detected.The pathological changes in periodontal tissues were observed.The expression levels of NLRP3 and Caspase-1 proteins were detected.Results The CEJ-AC distance,the expression levels of IL-6 and TGF-P in the serum,the positive rates of IL-6 and TGF-β expression in the periodontal tissues,and the expression levels of NLRP3 and Caspase-1 proteins were all increased in the model group(P<0.05).After the intervention with baicalein,all those indexes a-bove were decreased(P<0.05).The treatment of activator significantly attenuated the effect of baicalein on above indexes.Conclusion Baicalein reduces the inflammation response,and controls alveolar bone resorption by inhibition of NLRP3/Caspase-1 pathway.

关 键 词：黄芩素 牙周炎大鼠 NLRP3/Caspase-1通路 牙槽骨吸收 

分 类 号：R781.41[医药卫生—口腔医学]