对氯苯酚胁迫下李氏禾的净化性能、生长状态及生理生化响应  

Purification performance,growth status,and physiological and biochemical responses of Leersia hexandra Swartz under 4-chlorophenol stress

作　　者：王超 王义安[1,2] 林华 覃辉[1] 甘淑萍 KONG Chhuon WANG Chao;WANG Yi′an;LIN Hua;QIN Hui;GAN Shuping;KONG Chhuon(College of Environmental Science and Engineering,Guilin University of Technology,Guilin 541000,China;Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Areas,Guilin University of Technology,Guilin 541000,China;Faculty of Hydrology and Water Resources Engineering,Institute of Technology of Cambodia,Phnom Penh 12000,Cambodia)

机构地区：[1]桂林理工大学环境科学与工程学院,桂林541000 [2]桂林理工大学广西岩溶地区水污染控制与用水安全保障协同创新中心,桂林541000 [3]柬埔寨理工学院水文与水资源工程系,金边12000

出　　处：《应用与环境生物学报》2022年第3期662-667,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金面上项目(52070051);广西自然科学基金项目(2020GXNSFAA297256);广西高等学校高水平创新团队及卓越学者计划项目(桂财教函[2018]319);广西八桂学者和特聘专家项目资助。

摘　　要：研究不同浓度(0、1、5、10、25、50和75 mg/L)李氏禾(Leersia hexandra Swartz)在对氯苯酚(4-chloro phenol,4-CP)污染水体的净化效果及植物的生长状态和生理生化响应,揭示该植物对4-CP胁迫的耐受性等特性.结果显示:李氏禾对污水中的4-CP具有较好的净化去除作用,在大于10 mg/L 4-CP浓度下去除率有所下降但仍可达95%以上.低浓度(<5 mg/L)4-CP胁迫能够促进李氏禾生长,而高浓度(>5 mg/L)胁迫对植物生长具有明显的抑制作用.随着4-CP浓度增加,叶组织中可溶性蛋白含量下降至8.23 mg/g,丙二醛(MDA)和过氧化氢(H_(2)O_(2))含量显著升高,分别升高到6.03 nmol/mg(prot)、31.1 mmol/mg(prot),超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性分别先升到66.45 U/mg(prot)、25.33 U/mg(prot)后降至51.92 U/mg(prot)、17.68 U/mg(prot),过氧化物酶(POD)活性上升,达到55.80 U/mg(prot).上述结果表明李氏禾对4-CP胁迫具有一定的耐受性和净化作用,可作为4-CP污染水体的一种潜在可利用修复植物,结果可为后续采用植物修复方法治理酚类污染水体的研究提供依据.(图6参42)The purification effect of Leersia hexandra Swartz on water body stress at different concentrations(0,1,5,10,25,50,and 75 mg/L)of 4-chlorophenol(4-CP)pollution,as well as the growth status and physiological and biochemical responses of the plants were investigated to determine the tolerance and metabolic characteristics of L.hexandra under 4-CP stress.The results showed that L.hexandra had an effective purification and removal effect on 4-CP in sewage,and the removal rate of 4-CP dropped at concentrations greater than 10 mg/L,but could still reach more than 95%.Low concentrations(<5 mg/L)of stress promoted the growth of L.hexandra.In contrast,high-stress concentrations(>5 mg/L)had an inhibitory effect on plant growth.With the increase in 4-CP concentration,the soluble protein content in leaf tissues decreased to 8.23 mg/g and the levels of malondialdehyde and H_(2)O_(2) increased significantly to 6.03 nmol/mg prot and 31.1 mmol/mg prot respectively,and the activities of superoxide dismutase and catalase first increased to 66.45 U/mg prot and 25.33 U/mg prot and then decreased to 51.92 U/mg prot and 17.68 U/mg prot,respectively.Peroxidase activity increased to 55.80 U/mg prot.This study indicates that L.hexandra has a certain tolerance and purification effect under 4-CP stress and can be used as a potential phytoremediator for water bodies polluted by 4-CP.These results also provide a basis for follow-up studies on the treatment of phenolic-polluted water using phytoremediation.

关 键 词：李氏禾 对氯苯酚 生理生化 植物修复 

分 类 号：X173[环境科学与工程—环境科学]