七氟烷全身麻醉对失血性休克复苏小鼠血流动力学及记忆功能的影响机制  

Effects of Sevoflurane General Anesthesia on Hemodynamics and Memory Function in Hemorrhagic Shock Resuscitation Mice

作　　者：蒋中晋 种朋贵 李星辉 龙旭 雷定才 熬胜福 JIANG Zhong-jin;ZHONG Peng-gur;LI Xing-hui;LONG Xu;LEI Ding-cai;AO Sheng-fu(Department of Anesthesiology,The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,550001,China)

机构地区：[1]贵州中医药大学第二附属医院麻醉科,贵州贵阳550001

出　　处：《现代生物医学进展》2022年第10期1821-1824,1888,共5页Progress in Modern Biomedicine

基　　金：国家卫生健康委"十三五"规划全国重点项目(YYWS3176)。

摘　　要：目的:探讨七氟烷全身麻醉对失血性休克复苏小鼠血流动力学及记忆功能的影响机制。方法:将建立成功的42只失血性休克复苏小鼠按照随机数字表法平分为3组-模型组、氧气组、七氟烷组,对照组吸入空气4 h,氧气组吸入100.0%氧气4 h,七氟烷组吸入100.0%氧气和2.5%七氟烷4 h,监测与记录小鼠血流动力学及记忆功能变化情况。结果:氧气组、七氟烷组麻醉后第3 d与第7 d的Morris水迷宫逃避潜伏期少于模型组,穿越原平台次数多于模型组(P<0.05),氧气组与七氟烷组对比差异有统计学意义(P<0.05)。氧气组、七氟烷组麻醉后第3 d与第7 d的去甲肾上腺素(Norepinephrine,NE)pD_(2)与Emax值高于模型组(P<0.05),七氟烷组高于氧气组(P<0.05)。氧气组、七氟烷组麻醉后第3 d与第7 d的脑组织凋亡指数低于模型组(P<0.05),七氟烷组低于氧气组(P<0.05)。氧气组、七氟烷组麻醉后第3 d与第7 d的海马组织Akt、Caspase-3蛋白相对表达水平低于模型组(P<0.05),七氟烷组低于氧气组(P<0.05)。结论:七氟烷全身麻醉在失血性休克复苏小鼠的应用能抑制Akt、Caspase-3蛋白的表达,可抑制脑组织神经细胞凋亡,可改善血流动力学状况,促进小鼠记忆能力的恢复。Objective:To investigate the effect of sevoflurane general anesthesia on hemodynamics and memory function in mice resuscitated from hemorrhagic shock.Methods:The 42 successfully established hemorrhagic shock resuscitation mice were divided into 3groups according to the random number table method:model group,oxygen group and sevoflurane group.The control group were inhaled air for 4 hours,and the oxygen group inhaled 100.0% oxygen for 4 h,the sevoflurane group inhaled 100.0% oxygen and 2.5% sevoflurane for 4 h.The changes of hemodynamics and memory function of mice were monitored and recorded.Results:The escape latency of Morris water maze in oxygen group and sevoflurane group were less than that in model group(P<0.05),and the number of crossing the original platform were more than that in model group(P<0.05).There were significant difference compared between oxygen group and sevoflurane group(P<0.05).The PD_(2)and Emax values of norepinephrine(NE)in oxygen group and sevoflurane group were higher than those in model group(P<0.05),and those in sevoflurane group were higher than those in oxygen group(P<0.05).The apoptosis index of brain tissue in oxygen group and sevoflurane group were lower than that in model group(P<0.05),and that in sevoflurane group were lower than that in oxygen group(P<0.05).The relative expression levels of Akt and caspase-3 protein in hippocampus of oxygen group and sevoflurane group were lower than those of model group(P<0.05),and sevoflurane group were lower than that of oxygen group(P<0.05).Conclusion:Sevoflurane general anesthesia in hemorrhagic shock resuscitation mice can inhibit the expression of Akt and caspase-3 protein,inhibit neuronal apoptosis in brain tissue,improve hemodynamics and promote the recovery of memory ability in mice.

关 键 词：七氟烷 全身麻醉 失血性休克 复苏 小鼠 记忆功能 血流动力学 

分 类 号：R-33[医药卫生] R614R605.971