黄瓜果实CsMYB62克隆及其对CsGR-RBP3表达的调控  

Cloning of CsMYB62 and Its Regulations on CsGR-RBP3 Expression in Cucumber Fruit

作　　者：王斌[1] 武春爽 汤冰琳 王光 何金明[1] 曲姗姗 朱世江[2] WANG Bin;WU Chunshuang;TANG Binglin;WANG Guang;HE Jinming;QU Shanshan;ZHU Shijiang(Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan,Guangdong 512005;College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642)

机构地区：[1]韶关学院英东生物与农业学院,广东韶关512005 [2]华南农业大学园艺学院,广东广州510642

出　　处：《核农学报》2022年第5期907-917,共11页Journal of Nuclear Agricultural Sciences

基　　金：广东省基础与应用基础研究基金(2020A1515010068);广东省教育厅青年创新人才项目(2018KQNCX231);韶关学院博士科研启动项目(99000602)。

摘　　要：本研究前期证实,低温诱导的CsGR-RBP3表达与黄瓜果实耐冷性密切相关,但调控其表达的具体机理尚不清楚。为鉴定调控CsGR-RBP3表达的MYB转录因子,本研究采用转录组测序,分析了黄瓜果实在5℃低温处理后的转录组变化,并通过共表达趋势分析,鉴定了与CsGR-RBP3表达趋势一致的MYB基因。结果发现,CsMYB62与CsGR-RBP3表达的相关性很高,且低温处理能诱导黄瓜果实CsMYB62和CsGR-RBP3表达,推测CsMYB62转录因子可能调控CsGR-RBP3表达。为进一步分析CsMYB62基因的功能,从黄瓜果皮中克隆了CsMYB62基因全长序列。结果显示,CsMYB62基因全长834 bp,编码277个氨基酸残基。CsMYB62蛋白含有SANT保守域和MYB DNA结合域,定位在细胞核,在进化过程中高度保守。CsMYB62蛋白具有转录激活活性,能直接绑定到CsGR-RBP3启动子,增强CsGR-RBP3启动子活性,表明CsMYB62通过直接调控CsGR-RBP3表达而增强黄瓜果实耐冷性。本研究结果丰富了MYB调控网络,为进一步解析CsMYB62功能奠定了理论基础。It was previously demonstrated that the expression of CsGR-RBP3 under low temperature was closely associated with the chilling tolerance of cucumber fruit.However,the regulation mechanisms of its expression are still unknown.To identify the MYB transcription factors which regulated CsGR-RBP3 expression,this study analyzed the transcriptomic changes of cucumber fruit after treatment under 5℃ were analyzed through RNA-seq.The expression patterns of MYB genes which were consistent with those of CsGR-RBP3 were identified by co-expression analysis.The results showed that CsMYB62 and CsGR-RBP3 showed similar expression pattern during cold treatment,and cold treatment induced the expression of both genes.Therefore,we speculated that CsMYB62 might regulate CsGR-RBP3 expression.To further analyze the function of CsMYB62,the full length of CsMYB62 cDNA was cloned from cucumber peels.The open reading frame of CsMYB62 was 834 bp in length,which encoded 277 amino acids.CsMYB62 protein contained SANT and MYB DNA binding domains,and distributed in the nucleus.Plant MYB62 proteins were highly conserved during evolution.Cucumber CsMYB62 could directly bind to CsGR-RBP3 promoter and activate promoter activity,suggesting that CsMYB62 enhanced chilling tolerance of cucumber fruit by directly activating CsGR-RBP3 expression.The results of this study stretched the MYB regulatory network and laid a foundation for further studying the function of CsMYB62.

关 键 词：采后黄瓜 耐冷性 MYB转录因子 富甘氨酸RNA结合蛋白 转录调控 

分 类 号：S642.2[农业科学—蔬菜学]