miR-1和miR-206在不同饲养方式AA肉仔鸡肌肉组织中的表达分析  

Expression Analysis of miR-1 and miR-206 in Muscle Tissues of AA Broilers with Different Feeding Methods

作　　者：吴秋雪 张伟伟[1,2] 常鑫 张磊 邵淑丽[1,2] 孙婴宁[1,2] Wu Qiuxue;Zhang Weiwei;Chang Xin;Zhang Lei;Shao Shuli;Sun Yingning(College of Life Science and Agroforestry,Qiqihar University,Qiqihar,161006;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar,161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,齐齐哈尔161006

出　　处：《基因组学与应用生物学》2022年第1期34-40,共7页Genomics and Applied Biology

基　　金：国家青年科学基金项目(31801148);黑龙江省自然科学基金项目(C2016057);黑龙江省教育厅基本业务专项(135209260);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201874)共同资助。

摘　　要：为研究miR-1和miR-206在不同饲养方式下AA肉仔鸡肌肉组织中的表达情况,本试验采用生物信息学方法比对了miR-1、miR-206在不同物种中的同源性,通过茎环结构实时定量RT-PCR法分析miR-1和miR-206在不同饲养方式下AA肉仔鸡的肌肉组织中的表达,从而初步探究其表达规律。结果表明,gga-miR-1和gga-miR-206成熟序列分别位于鸡基因组20号和3号染色体上;pre-gga-miR-1与eca、ptr、efu、tgu、ola同源性高达86%,pre-gga-miR-206与bta、cfa、hsa、mml、ptr、efu、xtr同源性高达88%;gga-miR-1和gga-miR-206在幼鸡腿肌中表达量高于笼养鸡和散养鸡,且笼养鸡中gga-miR-1和gga-miR-206表达量高于散养鸡,在散养鸡的翅肌和胸肌中表达量高于幼鸡和笼养鸡。表明不同饲养方式下miR-1和miR-206在不同组织表达不同,导致肉品质不同。本研究有助于了解不同饲养方式下miR-1和miR-206表达规律,养殖户可根据不同需求采取相应的饲养方式。In order to study the expression of miR-1 and miR-206 in the muscle tissue of AA broilers under different feeding methods,this study used bioinformatics methods to compare the homology of miR-1 and miR-206 in different species.The stem-loop real-time quantitative RT-PCR method was used to analyze the expression of miR-1 and miR-206 in the muscle tissue of AA broilers under different feeding methods,so as to initially explore their expression rules.The results showed that the mature sequences of gga-miR-1 and gga-miR-206 were located on chromosomes 20 and 3 of the chicken genome,respectively;pre-gga-miR-1 had high homology(86%)with eca,ptr,efu,tgu,and ola,pre-gga-miR-206 had 88%homology with bta,cfa,hsa,mml,ptr,efu,xtr;gga-miR-1 and gga-miR-206 were highly expressed in young chicken leg muscles.The expression levels of gga-miR-1 and gga-miR-206 in caged chickens were higher than those of free-range chickens,and the expression levels in the wing and breast muscles of free-range chickens were higher than those of young chickens and Cage chickens.It showed that miR-1 and miR-206 were expressed differently in different tissues under different feeding methods,resulting in different meat quality.This study was helpful to understand the expression patterns of miR-1 and miR-206 under different feeding methods,and farmers could adopt corresponding feeding methods according to different needs.

关 键 词：MIR-1 miR-206 肌肉组织 骨骼肌 饲养方式 

分 类 号：S831.2[农业科学—畜牧学]