IFN-α诱导大鼠抑郁模型行为学以及海马组织CAl区5-HTA2R表达水平变化  

Changes in the Behavioristics of IFN-α Induced Depression in Rats and in the Expression Level of 5-HTA2R in the CAl Region of Hippocampal Tissue

作　　者：徐柏郁 池红井 刘金霞[1] XU Baiyu(Chengde Medical University,Hebei Chengde 067000,China)

机构地区：[1]承德医学院,河北承德067000 [2]河北省承德市卫健委信息中心,河北承德067000 [3]广东省深圳市南山区妇幼保健院,广东深圳518000

出　　处：《河北医学》2022年第7期1057-1061,共5页Hebei Medicine

基　　金：河北省科技厅科技支撑项目,(编号:14277730D,11276103D-62)。

摘　　要：目的:通过检测不同方式制备大鼠抑郁症模型的行为学、海马组织学及其5-HTA2R表达水平,探讨抑郁症发生机制。方法:选择30只大鼠,随机数字法分为正常对照组、干扰素组和CUMS模型组,各10只。通过皮下注射IFN-α12周,完成大鼠抑郁症模型制备,并以传统的CUMS大鼠抑郁症模型作为对照,进行比对研究。记录大鼠干预措施实施不同时间的体重、旷场实验、糖水偏好实验结果和新环境抑制进食时间等行为学指标;HE染色和硫瑾染色观察大鼠海马组织学变化;免疫组化检测大鼠5-HTA2R表达水平变化情况。结果:与对照组比较,干扰素组和模型组在大鼠体重和各项行为学实验积分等均有统计学差异(P<0.05);与模型组比较,干扰素组新环境抑制进食时间显著延长,有统计学差异(P<0.05),但大鼠体重变化、旷场试验积分以及糖水偏爱性实验结果无统计学差异(P>0.05);随着干预措施持续时间延长,大鼠体重、糖水偏爱性实验以及旷场实验评分积分逐渐减少,新环境抑制进食时间逐渐延长,有统计学差异(P<0.05);HE染色和硫瑾染色均发现,模型组海马CAl区有明显损伤征象,模型组有明显损伤征象,其细胞数量减少,细胞间隙增大,细胞层数变少,稀疏,排列紊乱,细胞结构不完整,呈萎缩状态,部分细胞出现空泡化,细胞核变小、固缩,也可见胞核肿胀、淡染、碎裂,甚至脱失,呈现嗜酸性染色;干扰素组域模型组类似;与正常组相比,干扰素组海马CAl区5-HTA2R免疫反应阳性神经元数目减少,差异有显著性(71.6%vs56.3%,χ^(2)=4.85 P<0.05),与模型对照组比较,海马CAl区5-HTA2R受体免疫反应阳性神经元数目略有减少,但无统计学差异(52.6%vs56.3%,χ^(2)=0.32 P>0.05)。结论:干扰素能够通过减少海马CAl区5-HTA2R表达水平,可能导致大鼠抑郁症发生,为研发抑郁症模型制备策略提供理论基础。Objective:To investigate the mechanism of depression by examining the behavioural and hippocampal histology and its 5-HTA2R expression levels in different ways of preparing rat depression models.Methods:Thirty rats were selected and divided by random number method into normal group,interferon group and CUMS model group,10 rats each.The rat depression model was prepared by subcutaneous injection of IFN-α for 12 weeks,and the traditional CUMS rat depression model was used as a control for a comparative study.Behavioural indicators such as body weight,results of the absenteeism test,sugar-water preference test and the time of feeding inhibition in the new environment were recorded at different times of the intervention;histological changes in the hippocampus of rats were observed by HE staining and auxin staining;changes in the expression level of 5-HTA2R in rats were detected by immunohistochemistry.Results:Compared with the control group,there was a statistical difference between the interferon group and the model group in terms of rat body weight and various behavioral experiment scores(P<0.05);compared with the model group,the duration of the new environmental inhibition of feeding was significantly longer in the interferon group,and there was a statistical difference(P<0.05),but there was no statistical difference(P>0.05)in the rat body weight change,the score of the absenteeism test and the results of the sugar-water preference experiment.There was a statistically significant difference(P<0.05)when the duration of the intervention was prolonged,with a gradual decrease in body weight,scores in the sugar-water preference test and the score of the absenteeism test,and a gradual increase of the duration of inhibition of feeding in the new environment(P>0.05).Both HE staining and auxin staining showed that the hippocampal CAl region of the model group showed obvious signs of injury,and the model group showed obvious signs of injury,with reduced cell numbers,increased cell gaps,fewer cell layers,sparse,disorganized

关 键 词：干扰素-Α 抑郁症 模型 5-HTA2R 

分 类 号：R-332[医药卫生] R749.4