SP2509联合顺铂调控LSD1的表达而诱导骨肉瘤细胞的凋亡和自噬  

SP2509 Induces Apoptosis and Autophagy in Osteosarcoma Cells by Regulating LSD1 Expression in Combination with Cisplatin

作　　者：夏非 张海平[1] XIA Fei;ZHANG Haiping(The Second Affiliated Hospital of Nantong University/Nantong First People's Hospital,Jiangsu Nantong 226000,China)

机构地区：[1]南通大学第二附属医院/江苏省南通市第一人民医院骨科,江苏南通226000

出　　处：《河北医学》2022年第7期1061-1066,共6页Hebei Medicine

基　　金：江苏省南通市科技技术项目,(编号:JC2021177)。

摘　　要：目的:探究SP2509联合顺铂对骨肉瘤细胞凋亡和自噬的影响及机制。方法:qRT-PCR检测LSD1在骨肉瘤细胞MG-63、HuO9、U-2OS以及人成骨细胞hFoB1.19中的表达。CCK8法检测SP2509和顺铂的IC50值。MG-63细胞分为对照组、SP2509组(20μmoL/L)、顺铂组(10μmoL/L)、SP2509(20μmoL/L)联合顺铂组(10μmoL/L),流式细胞术检测各组细胞凋亡水平,Western blot检测各组细胞LSD1、LC3-Ⅱ、P62、Caspase3、Bcl-2表达。结果:LSD1在骨肉瘤细胞MG-63、HuO9、U-2OS中的表达显著高于其在hFoB1.19中的表达,MG-63中LSD1表达最高。SP2509和顺铂IG50值分别为40和20μmoL/L。相比于对照组,SP2509组和顺铂组MG-63细胞凋亡水平显著增加(P<0.05),Caspase 3表达显著增加(P<0.05),Bcl-2表达显著下调(P<0.05),LC3-Ⅱ表达显著增加(P<0.05),P62表达显著下调(P<0.001),LSD1表达显著下调(P<0.05)。SP2509联合顺铂组细胞凋亡水平显著高于SP2509组和顺铂组(P<0.05),Caspase 3和LC3-Ⅱ表达显著高于SP2509组和顺铂组(P<0.05),Bcl-2、P62、LSD1表达显著低于SP2509组和顺铂组(P<0.05)。结论:SP2509联合顺铂可通过调控LSD1的表达而诱导骨肉瘤细胞的凋亡和自噬。Objective:To investigate the effect and mechanism of SP2509 combined with cisplatin on apoptosis and autophagy of osteosarcoma cells.Methods:The expression of LSD1 in osteosarcoma cells MG-63,HuO9,U-2OS and human osteoblast hFoB1.19 were detected by qRT-PCR.The IC50 values of SP2509 and cisplatin were detected by CCK8 method.MG-63 cells were divided into control group,SP2509 group(20μmoL/L),cisplatin group(10μmoL/L),SP2509(20μmoL/L)combined with cisplatin group(10μmoL/L),the level of apoptosis was detected by flow cytometry,and the expressions of LDSS1,LC3-Ⅱ,p62,caspase 3 and Bcl-2 were detected by Western blot.Results:The expression of LSD1 in osteosarcoma cells MG-63,HuO9 and U-2OS was significantly higher than that in hFoB1.19,and the expression of LSD1 was the highest in MG-63.The IG50 values of SP2509 and cisplatin were 40 and 20μmoL/L.Compared with the control group,the apoptosis level of MG-63 cells in SP2509 group and cisplatin group increased significantly(P<0.05),the expression of caspase 3 increased significantly(P<0.05),the expression of Bcl-2 decreased significantly(P<0.05),the expression of LC3-Ⅱ increased significantly(P<0.05),the expression of p62 decreased significantly(P<0.05),and the expression of LSD1 decreased significantly(P<0.05).The level of apoptosis in SP2509 combined with cisplatin group was significantly higher than that in SP2509 group and cisplatin group(P<0.05),the expression of Caspase 3 and LC3-Ⅱ was significantly higher than that in SP2509 group and cisplatin group(P<0.05),and the expression of Bcl-2,P62 and LSD1 was significantly lower than that in SP2509 group and cisplatin group(P<0.05).Conclusion:SP2509 combined with cisplatin can induce apoptosis and autophagy of osteosarcoma cells by regulating the expression of LSD1.

关 键 词：SP2509联合顺铂 骨肉瘤 凋亡 自噬 

分 类 号：R738.1[医药卫生—肿瘤]